FEBS Letters | |
Mechanism of chain initiation by dextransucrase | |
Parnaik, Veena K.1  Mayer, Robert M.1  | |
[1] Department of Chemistry, The Ohio State University, Columbus, OH 43210, USA | |
关键词: Dextransucrase; Polysaccharide synthesis; Chain initiation; | |
DOI : 10.1016/0014-5793(82)80794-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Dextran was synthesized using dextransucrase from Streptococus sanguis 10558 and (F)-[14C]sucrose as substrate to test the possibility that sucrose may be the initial acceptor for glucose. If sucrose is the initial acceptor, then dextran chains should have [14C] fructose in a terminal ‘sucrose’ linkage which can be cleaved under mild conditions. Although incorporation of [14C]fructose into dextran was observed, the label was not released by mild hydrolysis, indicating that sucrose is not the initiator for dextran synthesis. Incorporation of [14C]fructose into dextran might represent its ability to act as an acceptor, as suggested by the isolation of leucrose as a by-product in the reaction.
【 授权许可】
Unknown
【 预 览 】
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