期刊论文详细信息
FEBS Letters
Mechanism of chain initiation by dextransucrase
Parnaik, Veena K.1  Mayer, Robert M.1 
[1] Department of Chemistry, The Ohio State University, Columbus, OH 43210, USA
关键词: Dextransucrase;    Polysaccharide synthesis;    Chain initiation;   
DOI  :  10.1016/0014-5793(82)80794-1
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

Dextran was synthesized using dextransucrase from Streptococus sanguis 10558 and (F)-[14C]sucrose as substrate to test the possibility that sucrose may be the initial acceptor for glucose. If sucrose is the initial acceptor, then dextran chains should have [14C] fructose in a terminal ‘sucrose’ linkage which can be cleaved under mild conditions. Although incorporation of [14C]fructose into dextran was observed, the label was not released by mild hydrolysis, indicating that sucrose is not the initiator for dextran synthesis. Incorporation of [14C]fructose into dextran might represent its ability to act as an acceptor, as suggested by the isolation of leucrose as a by-product in the reaction.

【 授权许可】

Unknown   

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