Lactose- and cellobiose-derived branched trisaccharides and a sucrose-containing trisaccharide produced by acceptor reactions of Weissella confusa dextransucrase

卷:190

Shi, Qiao ; Juvonen, Minna ; Hou, Yaxi ; Kajala, Ilkka ; Nyyssola, Antti ; Maina, Ndegwa Henry ; Maaheimo, Hannu ; Virkki, Liisa ; Tenkanen, Maija

Univ Helsinki

关键词: Dextransucrase;    Oligosaccharide;    Weissella;    Isomelezitose;    alpha-(1 -> 2)-Linkage;    Tandem mass spectrometry;    NMR;   

DOI  :  10.1016/j.foodchem.2015.05.090

学科分类：食品科学和技术

【 摘 要 】

Dextran-producing Weissella have received significant attention. However, except for maltose, the acceptor reactions of Weissella dextransucrases with different sugars have not been investigated. The action of recombinant Weissella confuse VIT E-90392 dextransucrase was tested with several potential acceptors, particularly, analogs lactose and cellobiose. The major acceptor products of both disaccharides were identified as branched trisaccharides, with a glucosyl residue alpha-(1 -> 2)-linked to the acceptor's reducing end. An additional product, isomelezitose (6(Fru)-alpha-Glcp-sucrose), was also produced when using lactose as an acceptor. This is the first report of the synthesis of isomelezitose by a dextransucrase. The NMR spectra of the three trisaccharides were fully assigned, and their structures were confirmed by selective enzymatic hydrolysis. The trisaccharides prepared from C-13(6)glc sucrose and lactose were analyzed by ESI-MSn, and the fragmentation patterns of these compounds were characterized. (C) 2015 Elsevier Ltd. All rights reserved.