DNA‐dependent ATPase B of FM3A cells

FEBS Letters

Enomoto, Takemi¹  Hanaoka, Fumio¹  Watanabe, Yoshinari¹  Yamada, Masa-atsu¹  Nagata, Kyosuke¹  Tawaragi, Yasunori¹ 

[1] Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113, Japan

关键词: DNA-dependent ATPase B;    FM3A cells;    DNA polymerase α;    Separation;    Glycerol gradient centrifugation;    Low salt condition;   

DOI  :  10.1016/0014-5793(82)81067-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

One form of DNA-dependent ATPase (DNA-dependent ATPase B) has been purified from FM3A cells. In this report, we describe the association of DNA polymerase α activity with DNA-dependent ATPase B through a series of purification steps and the final separation of the two enzymes by glycerol gradient centrifugation operated at a low salt concentration.

【 授权许可】

Unknown