MSSP, a protein binding to an origin of replication in the c‐myc gene, interacts with a catalytic subunit of DNA polymerase α and stimulates its polymerase activity

FEBS Letters

Iguchi-Ariga, Sanae M.M.¹  Ariga, Hiroyoshi³  Galli, Ivo²  Niki, Takeshi³ 

[1] College of Medical Technology, Hokkaido University, Kita-ku, Sapporo 060-0812, Japan;Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305-5324, USA;Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo 060-0812, Japan

关键词: MSSP;    DNA polymerase α;    c-myc;   

DOI  :  10.1016/S0014-5793(00)01679-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

MSSP has been identified as a protein that binds to both single- and double-stranded sequences of a putative DNA replication origin sequence in the human c-myc gene. MSSP possesses versatile functions, including stimulation of DNA replication, transcriptional regulation, apoptosis induction, and cell transformation coordinated by c-Myc. MSSP contains two RNP domains, RNP1-A and RNP1-B, both of which are necessary for all of the functions of MSSP. In this study, we found that MSSP binds to the N-terminal region of a catalytic subunit of a human DNA polymerase α via its RNP domains both in vitro and in human cells. Furthermore, MSSP was released from the putative DNA replication origin of the c-myc gene after it complexed with DNA polymerase α, and MSSP stimulated DNA polymerase activity in vitro.

【 授权许可】

Unknown