Association of cap binding protein‐related polypeptides with cytoplasmic RNP particles of chick embryonic muscle

FEBS Letters

Lee, Kevin A.W.¹  Chakraborty, Dipak³  Darveau, Andre¹  Sonenberg, Nahum¹  Mukherjee, Asok K.³  Sarkar, Satyapriya² 

[1] Department of Biochemistry, McGill University, Montreal, Quebec H3G, Y6, Canada;Department of Neurology, Harvard Medical School, Boston, MA 02114, USA;Department of Muscle Research, Boston Biomedical Research Institute, Boston, MA 02114, USA

关键词: Cap binding protein;    Monoclonal antibody;    Cytoplasmic RNP particle;    CBP;    cap binding proteins;    mRNP;    messenger ribonucleoprotein;    2D-PAGE;    two-dimensional polyacrylamide gel electrophoresis;    SDS;    sodium dodecyl sulfate;    iRNP;    translation inhibitory ribonucleoprotein;   

DOI  :  10.1016/0014-5793(82)81064-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cap binding protein (CBP)-related polypeptides were identified in different cytoplasmic RNP particles of embryonic chick muscles using monoclonal antibody to purified CBP. A single immunoreactive peptide (M _r 78000) was present in preparations of both free mRNP particles and a novel 10 S translation inhibitory RNP particle. In contrast, proteins isolated from these particles showed two new low-M _r immunoreactive peptides (M _r 43000 and M _r 29000). No CBP related protein could be detected in polysomal mRNP, although an immunoreactive M _r 43000 CBP-related protein was present in polysomes. The relevance of the association of different CBP-related polypeptides with cytoplasmic RNP particles and polysomes are discussed.

【 授权许可】

Unknown