A new family of cytokinin receptors from Cereales

FEBS Letters

Zagranichnaya, T.K³  Selivankina, S.Yu²  Boziev, Kh.M³  Lipkin, V.M³  Kulaeva, O.N²  Hall, M¹  Brovko, F.A³  Karavaiko, N.N²  Zemlyachenko, Ya.V² 

[1] Institute of Biological Sciences, The University of Wales, Aberystwyth, Dyfed SY23 3DA, UK;Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, Moscow 127276, Russia;Pushchino Branch of Ovchinnikov-Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Moscow region 142292, Russia

关键词: Cytokinin-binding protein;    Anti-idiotype antibody;    Monoclonal antibody;    Chromatin;    RNA polymerase I;    Aba-i;    anti-idiotype antibodies;    Abz;    antibodies against trans-zeatin;    Ado-T;    adenosine-Toyopearl;    BA;    6-benzyladenine;    CBP;    cytokinin-binding protein;    mAb;    monoclonal antibody;    ZBP;    zeatin-binding protein;    Z-Sepharose;    trans-zeatin Sepharose;    Zr-T;    zeatin riboside-Toyopearl;   

DOI  :  10.1016/S0014-5793(98)00101-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The highly specific recognition of a natural cytokinin, trans-zeatin, by cytokinin-binding protein (CBP) of 67 kDa from barley leaves was detected with an assay developed on the basis of cytokinin competition in ELISA with anti-idiotype antibodies (raised against antibodies to zeatin) for complex formation with CBP. Monoclonal antibodies (mAbs) raised against 70 kDa CBP from etiolated maize seedlings cross-reacted with barley 67 kDa CBP and prevented barley CBP and trans-zeatin induced activation of transcription elongation directed by RNA polymerase I associated with barley chromatin. One mAb (Z-6) had an agonistic effect. Maize CBP replaced barley CBP in activation of RNA synthesis with cytokinin in the barley transcription system. Hence, a new family of cytokinin receptors with common functions and immunodeterminants including maize and barley CBPs was found.

【 授权许可】

Unknown