Synthesis of β‐glucogallin from UDP‐glucose and gallic acid by an enzyme preparation from oak leaves

FEBS Letters

Gross, G.G.¹ 

[1]Universität Ulm, Abteilung Allgemeine Botanik, 7900 Ulm, FRG

关键词: β-glucogallin (1-galloyl-β-D-glucose);    Gallic acid;    Uridine-5'-diphosphoglucose;    Esterification;    Cell-free extracts;    Quercus robur;   

DOI  :  10.1016/0014-5793(82)81244-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cell-free extracts from oak leaves were found to catalyze the esterification of ¹⁴C-labeled gallic acid and UDP-glucose. The product of the enzymatic reaction was identified as β-glucogallin by means of TLC and HPLC. The reaction proceded linearly with respect to time and protein concentration and had a pH-optimum at 7.0. Normal Michaelis-Menten kinetics were observed for the substrates gallic acid (K _M = 1.1 mM) and UDP-glucose (2.3 mM). The new enzyme described here can be classified as UDP-glucose: gallate glucosyltransferase, and it is assumed that it catalyzes the first step in the biosynthesis of gallotannins.

【 授权许可】

Unknown