Phenolic contents, antioxidant and anticholinesterase potentials of crude extract, subsequent fractions and crude saponins from Polygonum hydropiper L

BMC Complementary and Alternative Medicine

Muhammad Imran¹  Sajjad Ahmad¹  Abdul Sadiq¹  Farhat Ullah¹  Jawad Ahmed²  Muhammad Junaid¹  Muhammad Ayaz¹ 

[1]Department of Pharmacy, University of Malakand, Khyber Pakhtoonkhwa 18000, KPK, Pakistan

[2]Institute of Basic Medical Sciences (IBMS), Khyber Medical University (KMU), Peshawar, Pakistan

关键词: Polygonum hydropiper L;    Gallic acid;    ABTS;    DPPH;   

Others  :  1220175
DOI  :  10.1186/1472-6882-14-145

 received in 2014-01-24, accepted in 2014-04-30,  发布年份 2014

【 摘 要 】

Background

We investigated Polygonum hydropiper L. (P. hydropiper) for phenolic contents, antioxidant, anticholinesterase activities, in an attempt to rationalize its use in neurological disorders.

Methods

Plant crude extract (Ph.Cr), its subsequent fractions: n-hexane (Ph.Hex), chloroform (Ph.Chf), ethyl acetate (Ph.EtAc), n-Butanol (Ph.Bt), aqueous (Ph.Aq) and saponins (Ph.Sp) were evaluated for 1,1-diphenyl,2-picrylhydrazyl (DPPH), 2,2-azinobis[3-ethylbenzthiazoline]-6-sulfonic acid (ABTS) free radical scavenging potential. Further, acetylcholinesterase (AChE) & butyrylcholinesterase (BChE) inhibitory activities were performed using Ellman's assay. Moreover, total phenolic contents of plant extracts were determined and expressed in mg of gallic acid equivalent per gram of dry sample (mg GAE/g dry weight).

Results

Among different fractions, Ph.Cr (90.82), Ph.Chf (178.16), Ph.EtAc (203.44) and Ph.Bt (153.61) exhibited high phenolic contents. All fractions showed concentration dependent DPPH scavenging activity, with Ph.EtAc 71.33% (IC₅₀ 15 μg/ml), Ph.Bt 71.40% (IC₅₀ 3 μg/ml) and Ph.Sp 71.40% (IC₅₀ 35 μg/ml) were most potent. The plant extracts exhibited high ABTS scavenging ability i.e. Ph.Bt (91.03%), Ph.EtAc (90.56%), Ph.Sp (90.84%), Ph.Aq (90.56%) with IC₅₀ < 0.01 μg/ml. All fractions showed moderate to high AChE inhibitory activity as; Ph.Cr, 86.87% (IC₅₀ 330 μg/ml), Ph.Hex, 87.49% (IC₅₀ 35 μg/ml), Ph.Chf, 84.76% (IC₅₀ 55 μg/ml), Ph.Sp, 87.58% (IC₅₀ 108 μg/ml) and Ph.EtAc 79.95% (IC₅₀ 310 μg/ml) at 1 mg/ml). Furthermore the BChE inhibitory activity was most prominent in Ph.Hex 90.30% (IC₅₀ 40 μg/ml), Ph.Chf 85.94% (IC₅₀ 215 μg/ml), Ph.Aq 87.62% (IC₅₀ 3 μg/ml) and Ph.EtAc 81.01% (IC₅₀ 395 μg/ml) fractions.

Conclusions

In this study, for the first time, we determined phenolic contents, isolated crude saponins, investigated antioxidant and anticholinestrase potential of P. hydropiper extracts. The results indicate that P. hydropiper is enriched with potent bioactive compounds and warrant further investigation by isolation and structural elucidation to find novel and affordable compounds for the treatment of various neurological disorders.

【 授权许可】

   
2014 Ayaz et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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【 参考文献 】

• [1]Farnsworth N, Akerele O, Bingel A, Soejarto D, Guo Z: Medicinal plants in therapy. Bull WHO 1985, 63(6):965-981.
• [2]Sharma R: Medicinal plants of India-An encyclopedia. Daya Publishing House, 110035 Delhi, India 2003, 46-47.
• [3]Foster S, James AD: A field guide to medicinal plants: Eastern and central North America. Peterson Field Guide Series, 2nd edition, Houghton Mifflin Park Avenue, New York 10003 1990, 241.
• [4]Chevallier A: The encyclopedia of medicinal plants. In Dorling Kindersley London. London: Andrew DK Publishing; 1996:185. ISBN 9-780751-303148
• [5]Peng ZF, Strack D, Baumert A, Subramaniam R, Goh NK, Chia TF, Ngin Tan S, Chia LS: Antioxidant flavonoids from leaves of Polygonum hydropiper L. Phytochemistry 2003, 62(2):219-228.
• [6]Chan Y-C, Wang M-F, Chen Y-C, Yang D-Y, Lee M-S, Cheng F-C: Long-term administration of polygonum multiflorum thunb: reduces cerebral ischemia-induced infarct volume in gerbils. Am J Chin Med 2003, 31(1):71-77.
• [7]Chen LW, Wang YQ, Wei LC, Shi M, Chan YS: Chinese herbs and herbal extracts for neuroprotection of dopaminergic neurons and potential therapeutic treatment of parkinsons disease. CNS Neurol Disord Drug Targets 2007, 6(4):273-281.
• [8]Li X, Matsumoto K, Murakami Y, Tezuka Y, Wu Y, Kadota S: Neuroprotective effects of Polygonum multiflorum on nigrostriatal dopaminergic degeneration induced by paraquat and maneb in mice. Pharmacol Biochem Behav 2005, 82(2):345-352.
• [9]Kumpulainen J, Salonen J: Natural antioxidants and anticarcinogens in nutrition, health and disease. R Soc Chem UK 1999, 240:178-187.
• [10]Shah SM, Ayaz M, Khan A-u, Ullah F, Shah a-u-HA, Iqbal H, Hussain S: 1,1-Diphenyl,2-picrylhydrazyl free radical scavenging, bactericidal, fungicidal and leishmanicidal properties of Teucrium stocksianum. Toxicol Ind Health 2013. doi:10.1177/0748233713487250
• [11]Halliwell B: Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet 1994, 344:721-724.
• [12]Kuhnan J: The flavonoids. A class of semi-essential food components; their role in human nutrition. World Rev Nutr Diet 1976, 24:117-191.
• [13]Barlow A, Susan M: Toxicological aspects of antioxidants used as food additives. In Food Antioxidants. Netherlands: Springer; 1990:253-307.
• [14]Kil HY, Seong ES, Ghimire BK, Chung IM, Kwon SS, Goh EJ, Hoe K, Kim MJ, Lim JD, Lee D, Yu CY: Antioxidant and antimicrobial activities of crude sorghum extract. Food Chem 2009, 115:1234-1239.
• [15]Shahidi F, Wanasundara P: Phenolic antioxidants. Crit Rev Food Sci Nutr 1992, 32:67-103.
• [16]Atta-ur R, Wahab A, Nawas S, Choudhary M: New cholinesterase inhibiting bisbenzylisoquinoline alkaloids from Cocculus pendulus. Chem Pharm Bull 2004, 52:802-806.
• [17]Ahmad W, Ahmad B, Ahmad M, Iqbal Z, Nisar M, Ahmad M: In vitro inhibition of acetylcholinesterase, buty-rylcholinesterase and lipoxygenase by crude extract of Myricaria elegans Royle. J Biol Sci 2003, 11:1046-1049.
• [18]Jann MW: Preclinical pharmacology of metriphonate. Pharmacotherapy 1998, 18:55-67.
• [19]Aisen PS, Davis KL: The search for disease-modifying treatment for Alzheimer’s Disease. Neurology 1997, 48:35-41.
• [20]Bachman DL, Wolf PA, Linn RT: Prevalence of dementia and probable senile dementia of the Alzheimer's type in the Framingham study. Neurology 1992, 42:115-119.
• [21]Voet D, Voet JG: Serine proteases. In Biochemistry. 2nd edition. USA: John Wiley and Sons; 1995:390.
• [22]Knapp MJ, Knopman DS, Solomon PR, Pendlebury WW, Davis CS, Gracon SI: A 30-week randomized controlled trial of high-dose tacrine in patients with Alzheimer’s disease.The Tacrine study group. J Am Med Assoc 1994, 271:985-991.
• [23]Schneider LJ: Treatment of Alzheimer’s disease with cholinesterase inhibitors. Clin Geriatr Med 2001, 17(2):337-58.
• [24]Heinrich M, Teoh HL: Galanthamine from snowdrop-the development of a modern drug against Alzheimer’s disease from local Caucasian knowledge. J Ethnopharmacol 2004, 92:147-162.
• [25]Perry NSL, Houghton PJ, Theobald A, Jenner P, Perry EK: In-vitro inhibition of human erythrocyte acetylcholinesterase by Salvia lavandulaefolia essential oil and constituent terpenes. J Pharm Pharmacol 2000, 52:895-902.
• [26]Konan AB, Datte JY, Yapo P: Nitric oxide pathway-mediated relaxant effect of aqueous sesame leaves extract (Sesamum radiatum Schum. & Thonn.) in the guinea-pig isolated aorta smooth muscle. BMC Complement Altern Med 2008, 8:23. BioMed Central Full Text
• [27]Khan FA, Ullah Z, Haider S: Phytochemicals screening and antimicrobial activities of selected medicinal plants of Khyber pakhtunkhwa Pakistan. Afr J Pharm Pharmacol 2011, 5(6):746-750.
• [28]Kim D, Jaeong S, Lee C: Antioxidant capicity of phenolic phytochemicals from various cultivars of plums. Food Chem 2003, 81:321-326.
• [29]Braca A, Tommasi ND, Bari LD, Pizza C, Politi M, Morelli I: Antioxidant principles from Bauhinia terapotensis. J Nat Prod 2001, 64(7):892-895.
• [30]Re R, Pellegrini N, Proteggente A, Pannala A, Yong M, Rice-Evas C: Antioxidant activity applying an improved FBTS radical cation decolorization assay. Free Rad Biol Med 1999, 26(9/10):1231-1237.
• [31]Trevisan MTS, Macedo FVV, Meent M, Rhee IK, Verpoorte R: Screening for acetylcholinesterase inhibitors from plants to treat Alzheimer's disease. Química Nova 2003, 26(3):301-304.
• [32]Classics Ellman GL, Courtney KD, Andres V, Featherstone RM: A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 1961, 7:88-95.
• [33]Aruoma O: Methodological considerations for characterizing potential antioxidant actions of bioactive components in food plants. Mutat Res 2003, 9(20):523-524.
• [34]Jadhav H, Bhutani K: Antioxidant properties of Indian medicinal plants. Phytother Res 2002, 16(8):771-773.
• [35]Yildirim A, Mavi A, Oktay M, Kara A, Algur O, Bilaloglu V: Comparison of antioxidant and antimicrobial activities of tilia (Tilia argenta Desf Ex DC), sage (Salvia triloba L.) and black tea (Camellia sinensis) extracts. J Agric Food Chem 2000, 48(10):5030-5034.
• [36]Hasan S, Hossain M, Akter R, Jamila M, Mazumder M, Rahman S: DPPH free radical scavenging activity of some Bangladeshi medicinal plants. J Med Plants Res 2009, 3(11):875-879.
• [37]Saeed S, Urfi M, Ali T, Khimani F, Gilani A: Antioxidants: their role in health and disease. Int J Pharmacol 2005, 1(3):210-217.
• [38]Bashir S, Gilani A: Studies on the antioxidant and analgesic activities of Aztec marigold (Tagetes erecta) flowers. Phytother Res 2008, 22(12):1692-1694.
• [39]Soare J, Dinis T, Cunha A, Almeida L: Antioxidant activities of some extracts of Thymus zygis. Free Radic Res 1997, 26(5):469-478.
• [40]Hagerman A, Riedl K, Jones G, Sovik K, Ritchard N, Hartzfeld P: High molecular weight plant polyphenolics (Tannins) asbiological antioxidants. J Agric Food Chem 1998, 46:1887-1892.
• [41]Ingkaninan K, Temkitthawon P, Chuenchon K, Yuyaem T, Thongnoi W: Screening for acetylcholinesterase inhibitory activity in plants used in Thai traditional rejuvenating and neurotonic remedies. J Ethnopharmacol 2003, 89:261-264.
• [42]Tildesley NTJ, Kennedy DO, Perry EK, Ballard CG, Savelev S, Sesnes KA, Scholey AB: Salvia lavandulaefolia (Spanish sage) enhances memory in healthy young volunteers. Pharmacol Biochem Behav 2003, 75:669-674.