Reconstitution of cytochrome bc 1 complex into lipid vesicles and the restoration of uncoupler sensitivity

FEBS Letters

Rich, Peter R.¹  Clarke, Sonia D.¹ 

[1] Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, England

关键词: Mitochondrial bc complex;    Lipid vesicle;    Respiratory control;    Uncoupler sensitivity;   

DOI  :  10.1016/0014-5793(82)81241-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Beef heart mitochondrial bc ₁ complex (ubiquinone—cytochrome c oxidoreductase) has been assayed by its ability to catalyse the reaction of duroquinol reduction of ferricytochrome c. When the isolated complex is reincorporated into lipid vesicles, the enzyme-catalysed electron transfer rate becomes uncoupler-sensitive. Initial experiments suggest that a protonmotive force is generated across the vesicles when electron transfer is initiated. Both ΔΨ and ΔpH components of this protonmotive force then influence an internal rate constant of the bc ₁ complex.

【 授权许可】

Unknown