FEBS Letters | |
Movement of amino acids into isolated plant mitochondria | |
Proudlove, Michael O.1  Moore, Anthony L.1  | |
[1] Biochemistry Department, School of Biological Sciences, University of Sussex, Falmer, Brighton BN1 9QG, Sussex, England | |
关键词: Plant mitochondria; Photorespiration; Centrifugal filtration; Transport characteristic; Amino acid; Keto acid; BSA; bovine serum albumin; CHCA; α-cyano-4-hydroxycinnamate; FCCP; carbonyl cyanide p-trifluoromethoxyphenylhydrazone; MOPS; 3-(N-morpholino)propanesulphonic acid; OAA; oxaloacetate; 2-OG; 2-oxoglutarate; PTA; phthalonic acid; PVP; polyvinylpolypyrrolidone; | |
DOI : 10.1016/0014-5793(82)81004-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Aspartate, glutamate, serine and glycine all permeate the inner membrane of mitochondria isolated from both etiolated and green plant tissues. No significant difference was found in the transport characteristics shown by mitochondria from either tissue. Influx of each amino acid appears diffusional because substrate saturation was not observed and there was no indication of specific inhibition or a requirement for a compensatory or counter ion for uptake. In contrast, uptake of the keto acid pyruvate did appear saturable. Inhibition by α-cyano-4-hydroxycinnamate, mersalyl and FCCP, but not valinomycin, suggests a carrier and a ΔpH mediate pyruvate transport into the matrix.
【 授权许可】
Unknown
【 预 览 】
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RO201912020283511ZK.pdf | 392KB | download |