期刊论文详细信息
FEBS Letters | |
Kirromycin‐resistant elongation factor Tu from wild‐type of Lactobacillus brevis | |
Wolf, Heinz1  Wörner, Walter1  | |
[1] Institut für Biologie II, Lehrstuhl für Mikrobiologie I der Universität, D-74000 Tübingen, FRG | |
关键词: Elongation factor Tu; Lactobacillus brevis; Kirromycin resistance; Pulvomycin; Nucleotide-binding site; Heat stability; EF; elongation factor; EF-Tuf; nucleotide-free elongation factor Tu; affices; refer to the source of the protein; Ec; Escherichia coli; Lb; Lactobacillus brevis; | |
DOI : 10.1016/0014-5793(82)80944-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Properties of the elongation factor Tu from Lactobacillus brevis which is naturally insensitive to kirromycin are described. The protein is characterized by an unusual nucleotide-binding site with increased affinity for GTP and extreme heat stability. EF-Tu is sensitive to pulvomycin in the assay of polyphenylalanine synthesis. However, the failure of the protein to display pulvomycin-dependent GDP-binding and GTPase activity indicates that pulvomycin action in L. brevis differs from that in E. coli.
【 授权许可】
Unknown
【 预 览 】
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