A translational inhibitor activated in rabbit reticulocyte lysates under high pO2

FEBS Letters

Almış-Kanıgür, Gönül¹  Kospançalı, Serpil¹  Bermek, Engin¹  Kan, Beki¹ 

[1] Department of Biophysics, Istanbul Medical Faculty, Çapa, Istanbul, Turkey

关键词: Translational inhibitor;    Reticulocyte lysates;    High pO2;    Hemin-controlled repressor;    Glutathione disulfide;    Glucose 6-phosphate;    hpO2;    high pO2;    hpO2I;    high pO2-induced inhibitor;    HCR;    hemin-controlled repressor;    GSSG;    glutathione disulfide;    GSSGI;    glutathione disulfide-activated inhibitor;    dsRNA;    double-stranded RNA;    PAI;    pressure-activated inhibitor;    eIF-2;    eukaryotic initiation factor 2;    G6P;    glucose 6-phosphate;   

DOI  :  10.1016/0014-5793(82)81223-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An inhibitor of protein synthesis was activated under high oxygen partial pressure (pO₂) in hemin-supplemented and glutathione disulfide-free lysates from rabbit reticulocytes. This inhibitor shared some common features with other translational inhibitors from rabbit reticulocytes; that is, hemin-controlled repressor, glutathione disulfide-activated inhibitor and high pressure-activated inhibitor. It caused biphasic kinetics of inhibition which could be potentiated by ATP. Its activation was prevented by cAMP or glucose 6-phosphate. The high pO₂-inhibitor could be partially purified from post-ribosomal supernatant containing ribosomal salt wash by precipitation between 0–50% (NH₄)₂SO₄-saturation, Sephadex G-100, and DEAE-cellulose chromatography.

【 授权许可】

Unknown