ZINC AND LOW-DOSE OF CADMIUM PROTECT SERTOLI CELLS AGAINST TOXIC-DOSE OF CADMIUM: THE ROLE OF METALLOTHIONEIN

Iranian journal of reproductive medicine

MODARRESSI MOHAMMAD HOSSEIN¹  NOURMOHAMMADI ISSA¹  AHMADI FAGHIH MOHAMMAD AMIN¹  KHERADMAND FATEMEH¹  FIROOZRAI MOHSEN¹ 

关键词: CADMIUM;    GENE EXPRESSION;    METALLOTHIONEIN;    SERTOLI CELLS;    ZINC;   

DOI  :  

学科分类：药学、药理学、毒理学（综合）

来源: Shahid Sadoughi University of Medical Sciences, Yazd

【 摘 要 】

Background: The impact of cadmium (Cd) on male infertility may be related to the interaction with metal-binding proteins known as metallothioneins (Mts). Trace elements like zinc (Zn) have protective effects on testicular damage induced by Cd.Objective: We determined the effect of Zn and low-dose Cd pre-treatment on the expression of Mt1 and Mt2 genes on testicular Sertoli cells.Materials and Methods: The cultured TM4 mouse sertoli cells were treated with 50 mM ZnSO4 (Zn pre-treated group; ZnPG), 2 ?M CdCl2 (Cd pre-treated group; CdPG), or distilled water (DW pre-treated group; DWPG). After 18 hour, all of these groups were exposed to 100 mM CdCl2 for different periods of time (1, 2, 3, and 6 hours). There was also a control group for all three groups, which was treated only with distilled water (without Cd or Zn pre-treatment). Cellular viability, Zn and Cd concentrations and gene expression were assessed by MTT, atomic absorption spectrometry and real time PCR methods, respectively.Results: The expression of Mt1 and Mt2 genes in ZnPG, CdPG, and DWPG was greater than the control group (p=0.02 and p=0.01, respectively). Cd concentrations in CdPG and DWPG were greater than the control group (p=0.00). Expression of both genes in ZnPG and CdPG increased after 3 hours of treatment and Cd concentration decreased simultaneously, which was more obvious in ZnPG.Conclusion: Zn and short term low-dose Cd pre-treatment might reduce the adverse effects of Cd by increasing expression of Mts genes in Sertoli cells. The protective effect of Zn was stronger than Cd.

【 授权许可】

Unknown