Compositions and Methods for Inhibiting Gene Expressions

Williams, Loren D [Inventor]Fang, Po-Yu [Inventor]Hsiao, Chiaolong [Inventor]Williams, Justin [Inventor]

关键词: RIBONUCLEIC ACIDS;    IN VIVO METHODS AND TESTS;    PACKAGING;    VIRUSES;    PURIFICATION;    ASSEMBLING;    GENE EXPRESSION;    PATENTS;    INVENTIONS;   

RP-ID  :  US-Patent-9,932,563, US-Patent-Appl-SN-14/911,645

学科分类：生物科学（综合）

美国|英语

来源: NASA Technical Reports Server

【 摘 要 】

A combined packing and assembly method that efficiently packs ribonucleic acid (RNA) into virus like particles (VLPs) has been developed. The VLPs can spontaneously assemble and load RNA in vivo, efficiently packaging specifically designed RNAs at high densities and with high purity. In some embodiments the RNA is capable of interference activity, or is a precursor of a RNA capable of causing interference activity. Compositions and methods for the efficient expression, production and purification of VLP-RNAs are provided. VLP-RNAs can be used for the storage of RNA for long periods, and provide the ability to deliver RNA in stable form that is readily taken up by cells.