EFFECTS OF PRENATAL EXPOSURE TO EXTREMELY LOW ELECTRO-MAGNETIC FIELD ON IN VIVO DERIVED BLASTOCYSTS OF MICE

Iranian journal of reproductive medicine

SOHOULI PARDIS¹  AMIRI SARA¹  GHANBARI ALI¹  BAYAT PARVIN DOKHT¹  DARABI MOHMMAD REZA¹ 

关键词: BLASTOCYST;    ELECTROMAGNETIC FIELDS;    MICE;   

DOI  :  

学科分类：药学、药理学、毒理学（综合）

来源: Shahid Sadoughi University of Medical Sciences, Yazd

【 摘 要 】

Background: Indisputable population exposure to widespread electromagnetic fields, has grown concerns over the probable health effects of these fields.Objective: The present study was aimed to examine the possible effects of 50 Hz extremely low frequency electromagnetic field (ELF-EMF) exposure on the number and quality of mice blastocysts.Materials and Methods: In present study, 66 NMRI pregnant females divided into two treated and non-treated groups. The treated group exposed to ELF-EMF (50 Hz and 6�?10-3 T). Subsequently, embryos were collected by flushing the uterine horn and Fallopian tubes on the day 3 of gestation. Number of trophoectoderm (TE) and Inner Cell Mass (ICM) cells in blastocysts were determined after differential nuclei staining using a modified method. Furthermore, number of all flushed blastocysts calculated in each group.Results: There was no significant difference in mean number of blastocysts in treated (6.64±1.34) and none treated (8.22±1.59) groups. In treated group, there were significant decreased in total cell number of blastocysts (p=0.000), number of ICM cells (p=0.000), and number of TE cells (p=0.001) whereas the ratio of ICM/TE cells increased (p=0.002).Conclusion: The data indicate that ELF-EMF is able to affect cellular composition of blastocysts, but it can't omit total volume of blastocysts.

【 授权许可】

Unknown