ANTISPASMODIC EFFECT OF PHYSALIS ALKEKENGI FRUIT EXTRACT ON RAT UTERUS

Iranian journal of reproductive medicine

GHARIB NASERI ZAHRA¹  GHARIB NASERI MOHAMMAD KAZEM¹  MOHAMMADIAN M.¹ 

关键词: RAT;    UTERUS;    PHYSALIS ALKEKENGI;    ANTISPASMODIC;   

DOI  :  

学科分类：药学、药理学、毒理学（综合）

来源: Shahid Sadoughi University of Medical Sciences, Yazd

【 摘 要 】

Background: Studies have shown that Physalis alkekengi reduces implantation and induces antifertility in rat. In Iranian traditional medicine it is believed that this plant has abortifacient and antifertility activities.Objective: The goal of this study was to evaluate the effect of Physalis alkekengi ripe fruit hydroalcoholic extract (PFE) on uterine contractility and its possible mechanism(s).Materials and Methods: Extraction of Physalis alkekengi fruit was carried out by maceration method (70% alcohol). Uterus was dissected out from adult non-pregnant rat (Wistar) and contracted by KCl (60mM) or oxytocin (10mU/ml) in an organ bath containing De Jalon solution and the effect of PFE on the uterine contractions was investigated. Furthermore, the role of a- and b-adrenoceptors, opioid receptors, nitric oxide and cyclic guanosine monophosphate synthesis inhibitors on the extract effects were evaluated.Results: KCl- and oxytocin-induced uterine contractions were inhibited (p<0.001) by the cumulative concentrations of the extract in a concentration dependent manner.Incubation of uterus with propranolol (1mM) and L-NAME (100mM) attenuated the PFE antispasmodic effect (p<0.05). But the PFE effect was unaffected by phentolamine (1mM), naloxone (1mM) or methylene blue (10mM). In Ca2+-free with high potassium (60mM) De Jalon solution, cumulative concentrations of CaCl2 (0.1-0.5mM) induced uterine contraction concentration-dependently (p<0.001). Uterus incubation (5min) with PFE (0.25-1.75mg/ml) attenuated the CaCl2�?�induced contractions (p<0.05).Conclusion: It seems that the extract induced antispasmodic effect mainly via calcium influx blockade and partially through blocking b-adrenoceptors and nitric oxide (NO) synthesis. However, neither a-adrenoceptors nor opioid receptors or cGMP synthesis were involved.

【 授权许可】

Unknown