THE EFFECT OF HYALURONIC ACID ON MOTILITY, VITALITY AND FERTILIZATION CAPABILITY OF MOUSE SPERMS AFTER CRYOPRESERVATION

Iranian journal of reproductive medicine

AKBARI M.¹  PASBAKHSH P.¹  BAKHTIARI MITRA¹  SOBHANI A.¹  HEDAYATPOUR AZIM¹  AMIDI F.¹  SARGOLZAEI AVAL F.¹  ABASI M.¹ 

关键词: HYALURONIC ACID (HA);    CRYOPRESERVATION;    IN VITRO FERTILIZATION (IVF);    SPERM PARAMETERS;   

DOI  :  

学科分类：药学、药理学、毒理学（综合）

来源: Shahid Sadoughi University of Medical Sciences, Yazd

【 摘 要 】

Background: Various approaches have been used in the attempts to improve the quality of frozen�?�thawed mouse sperms. According to literatures, it seems that hyaluronic acid (HA) has an important role on the permeability and motility of sperms and their interaction with gametes. Objective: For evaluation of HA supplementation on sperm characteristics and fertilization capability, we investigated the effect of different doses of HA on mouse sperm morphology, motility, vitality and fertilization capability after freezing and thawing. Materials and Methods: The cauda epididymes was removed from 6 male mice with aseptic method. The sperm samples were frozen in 1.8 ml cryotubes with 18% raffinose and 3% skimmed milk containing cryo-protectant solution. HA at the concentration of 750, 1000 or 1250 mg/ml was supplemented to frozen-thawed sperms. Sperm motility was measured with microscope, and fertilization rate was evaluated after routine IVF by counting the fertilized oocytes. For sperm morphology, papaniclau staining was used while; Eosin B was used for the assessment of sperm viability rate. Results: HA supplementation (750mg/ml) improved motility parameters (p<0.05) and increased the fertility rate (p<0.05). The effect of 1,000 mg/ml HA was also positive on the sperms. But 1,250 mg/ml HA had negative effect on above mentioned characteristic. On the other hand, none of these doses had any effect on sperm morphology.

【 授权许可】

Unknown