Ternary hybrid nanocomposites for gene delivery and magnetic resonance imaging of hepatocellular carcinoma cells

Quantitative Imaging in Medicine and Surgery

Josie M. Y. Lai⁴  Kathy W. Y. Sham⁴  Chun-Pong Chak¹  Ken Cham-Fai Leung²  Siu-Fung Lee¹  Chi-Hin Wong²  Xiao-Ming Zhu⁵  Christopher H. K. Cheng⁴  Yi-Xiang J. Wang³ 

[1] Department of Chemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, P. R. ChinaDepartment of Chemistry and Institute of Creativity, The Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR, P. R. China;;Department of Imaging and Interventional Radiology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, P. R. China;School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, P. R. China;Department of Chemistry and Institute of Creativity, The Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR, P. R. China;Department of Imaging and Interventional Radiology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, P. R. China

关键词: Deferoxamine;    gene delivery;    hepatocellular carcinoma (HCC);    magnetic resonance imaging (MRI);    superparamagnetic iron oxide (SPIO);   

DOI  :  10.3978/j.issn.2223-4292.2013.12.05

学科分类：外科医学

来源: AME Publications

【 摘 要 】

This paper describes comparative studies in magnetic resonance imaging (MRI) and gene deliveries toward hepatocellular carcinoma (HCC) HepG2 cells with ternary composites that consist of superparamagnetic iron oxide (SPIO) nanoparticles (NPs) (8-10 nm) with deferoxamine coating, circular plasmid DNA (~4 kb) equipped with green fluorescent probe, and branched polyethylenimine (PEI) (25 kDa, PDI 2.5). The packaging of the ternary complexes has been characterized by agarose gel retardation assay. By tuning the PEI/NP ratios and with a fixed DNA amount, different ternary composites have been employed for NP/gene transfection towards HepG2 cells, which have been characterized by in vitro MRI and green fluorescence protein (GFP) fluorescence.

【 授权许可】

Unknown