Evaluation of the Phytochemical and Antimicrobial potential of the Leaf Extracts of Bryophyllum pinnatum L.and Citrus aurantifolia Sw. and their Synergy.

Journal of Applied Sciences and Environmental Management

AKINNIBOSUN, F. I.¹  EDIONWE, O.¹ 

关键词: Bryophyllum pinnatum;    Citrus aurantifolia;    Synergy;    Antimicrobial;    Phytochemical constituents;   

DOI  :  

学科分类：环境科学（综合）

来源: University of Port Harcourt * Department of Pure and Industrial Chemistry

【 摘 要 】

The aim of this research is to comparatively study the phytochemical andantimicrobial properties of Bryophyllum pinnatumand Citrus aurantifolialeaf extracts andtheir synergy. Escherichia coli , Staphylococcus aureus , Klebsiella pneumoniae , and Pseudomonas aeruginosa , Aspergillus niger , Mucor mucedo , Penicillium notatumand Candida albicans , were used as test organisms and the antimicrobial activity of the extractswas determined by the agar-well diffusion method. Synergistic antibacterial activity rangedfrom 0.0 ± 0.0 to 2.4 ± 0.6, 11.3 ± 0.9 to 23.5 ± 1.1, 16.7 ± 0.3 to 27.3 ± 0.6 and 8.7 ± 0.9 to22.7 ± 0.9, for aqueous, ethanol, methanol and acetone extracts respectively. Extracts of C.aurantifolia were more effective against the test organisms than B. pinnatum extracts,except the aqueous extract. Synergistic antifungal activity of the aqueous extract was 0.0 ±0.0 mm for all the test fungi, the synergistic antifungal activity ranged from 8.7 ± 0.6 mm to14.0 ± 0.9 mm, 10.0 ± 0.9 mm to 21.7 ± 0.6 mm and 0.0 ± 0.0 mm to 20.0 ± 0.6 mm for theethanol, methanol and acetone extract respectively. Larger zones of inhibition wereobserved in the methanol extract of the synergy than the other extracting solvents. Thesynergy gave higher zones of inhibition neither B. pinnatum extract nor C. aurantifoliaextract could give. It was also observed that the extracts compared well with the standardantimicrobial agents used as positive control. The phytochemical analysis of the extractrevealed the presence of phytochemical constituents which conferred antimicrobial propertyon the plants. From the foregoing, the methanol extract of the synergy is considered themost effective in the treatment of infections caused by the test organisms.

【 授权许可】

Unknown