Antimicrobial and antioxidant activity of kaempferol rhamnoside derivatives from Bryophyllum pinnatum

BMC Research Notes

Pierre Tane⁵  Jules-Roger Kuiate³  Judit Hohmann¹  Peter Forgo¹  Dezső Csupor¹  Léopold Havyarimana⁴  Jean de Dieu Tamokou³  Simplice Joel Ndendoung Tatsimo² 

[1] Department of Pharmacognosy, University of Szeged, Eötvös u. 6, H-6720 Szeged, Hungary;Department of Chemistry, Higher Teachers' Training College, University of Maroua, P.O. Box 55, Maroua, Cameroon;Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon;Department of Chemistry, Faculty of Science, University of Burundi, P.O. Box 2700, Bujumbura, Burundi;Department of Chemistry, University of Dschang, PO Box 67, Dschang, Cameroon

关键词: Minimum inhibitory concentration;    Antioxidant;    Antimicrobial;    Kaempferol rhamnosides;    Crassulaceae;    Bryophyllum pinnatum;   

Others  :  1166561
DOI  :  10.1186/1756-0500-5-158

 received in 2011-12-01, accepted in 2012-03-20,  发布年份 2012

【 摘 要 】

Background

Bryophyllum pinnatum (Lank.) Oken (Crassulaceae) is a perennial succulent herb widely used in traditional medicine to treat many ailments. Its wide range of uses in folk medicine justifies its being called "life plant" or "resurrection plant", prompting researchers' interest. We describe here the isolation and structure elucidation of antimicrobial and/or antioxidant components from the EtOAc extract of B. pinnatum.

Results

The methanol extract displayed both antimicrobial activities with minimum inhibitory concentration (MIC) values ranging from 32 to 512 μg/ml and antioxidant property with an IC₅₀ value of 52.48 μg/ml. Its partition enhanced the antimicrobial activity in EtOAc extract (MIC = 16-128 μg/ml) and reduced it in hexane extract (MIC = 256-1024 μg/ml). In addition, this process reduced the antioxidant activity in EtOAc and hexane extracts with IC₅₀ values of 78.11 and 90.04 μg/ml respectively. Fractionation of EtOAc extract gave seven kaempferol rhamnosides, including; kaempferitrin (1), kaempferol 3-O-α-L-(2-acetyl)rhamnopyranoside-7-O-α-L-rhamnopyranoside (2), kaempferol 3-O-α-L-(3-acetyl)rhamnopyranoside-7-O-α-L-rhamnopyranoside (3), kaempferol 3-O-α-L-(4-acetyl)rhamnopyranoside-7-O-α-L-rhamnopyranoside (4), kaempferol 3-O-α-D- glucopyranoside-7-O-α-L-rhamnopyranoside (5), afzelin (6) and α-rhamnoisorobin (7). All these compounds, except 6 were isolated from this plant for the first time. Compound 7 was the most active, with MIC values ranging from 1 to 2 μg/ml and its antioxidant activity (IC₅₀ = 0.71 μg/ml) was higher than that of the reference drug (IC₅₀ = 0.96 μg/ml).

Conclusion

These findings demonstrate that Bryophyllum pinnatum and some of its isolated compounds have interesting antimicrobial and antioxidant properties, and therefore confirming the traditional use of B. pinnatum in the treatment of infectious and free radical damages.

【 授权许可】

   
2011 Tatsimo Ndendoung et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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