Interaction of Apidaecin Ib with Phospholipid Bilayers and its Edwardsiella Species-specific Antimicrobial Activity

Bulletin of the Korean chemical society

Min Jeong Lee¹  Ho Sung Moon¹  Hye-Jin Go¹  Tae Hyun Park¹  Bo-Hye Nam¹  Yong-Ki Hong¹  Hyun Do Jeong¹  Nam Gyu Park¹  Jung-Kil Seo¹ 

关键词: Apidaecin Ib;    Edwardsiella species;    Alternatives;    Fish pathogenic bacteria;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Apidaecin Ib had strong antimicrobial activity against several tested Gram-negative bacteria including Escherichia coli, Enterobacter cloacae, and Shigella flexneri (MECs; 0.3-1.5 μg/mL), but showed no activity against all the tested Gram-positive bacteria including Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus and one yeast, Candida albicans (MECs; >125 μg/mL). Interestingly, this peptide showed potent antibacterial activity only against Edwardsiella species (MECs; 0.6-3.6 μg/mL) among the tested fish pathogenic bacteria through a bacteriostatic process and showed no significant hemolytic activity. Apidaecin Ib took an unordered structure in all environments and also had very weak membrane perturbation activity even at 25 μM. Anti-Edwardsiella activity of apidaecin Ib is stronger than those of other antimicrobial polypeptides or antibiotics, but its activity is salt-sensitive. These results suggest that apidaecin Ib has Edwardsiella speciesspecific antibacterial activity and could be applied as new preventive or control additives for Edwardsiella species infection in freshwater fish aquaculture.

【 授权许可】

Unknown