Detection of Sequence-Specific Gene by Multi-Channel Electrochemical DNA Chips

Bulletin of the Korean chemical society

Jie Huang¹  Zhengguo Cui¹  Xuzhi Zhang¹  Bing Yang¹  Xinming Ji¹ 

关键词: DNA chips;    WSSV gene;    Multi-channel electrochemical detection;    Electroanalysis;    Loop-mediated isothermal amplification;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Five-channel electrochemical chips were fabricated based on the Micro-electromechanical System (MEMS) technology and were used as platforms to develop DNA arrays. Different kinds of thiolated DNA strands, whose sequences were related to white spot syndrome virus (WSSV) gene, were separately immobilized onto different working electrodes to fabricate a combinatorial biosensor system. As a result, different kinds of target DNA could be analyzed on one chip via a simultaneous recognition process using potassium ferricyanide as an indicator. To perform quantitative target DNA detection, a limit of 70 nM (S/N=3) was found in the presence of 600 nM coexisting noncomplementary ssDNA. The real samples of loop-mediated isothermal amplification (LAMP) products were detected by the proposed method with satisfactory result, suggesting that the multichannel chips had the potential for a high effective microdevice to recognize specific gene sequence for pointof- care applications.

【 授权许可】

Unknown