Ultra-Specific Enrichment of GST-Tagged Protein by GSH-Modified Nanoparticles

Bulletin of the Korean chemical society

Arudra Palani¹  Minsik Kim¹  Kwangyeol Lee¹  Je Sun Lee¹  Sang Won Lee¹  Jiyoung Huh¹  Jong Moon Park¹  Yeon Ji Lee¹ 

关键词: Proteomics;    Glutathione;    Superparamagnetic;    Nanoparticle;    Separation;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

The selective isolation of specific proteins from complex protein mixtures by nanoparticles is reported. Glutathionemodified superparamagnetic nanoparticles were used to purify specific proteins fused with glutathione S-transferase via enzyme-substrate interactions. They demonstrated greatly improved selectivity and efficiency over micron sized capturing beads. The ultra-specific enrichment of target proteins was confirmed by both SDS-PAGE and LC/ MS/MS experiments.

【 授权许可】

Unknown