Construction of 1H-15N Double Resonance Solid-State NMR Probefor Membrane Proteins in Aligned Bicelles

Bulletin of the Korean chemical society

Ji Sun Kim¹  Seung Hoon Um¹  Tae Joon Park¹  Yongae Kim¹ 

关键词: Solid-state NMR;    Home-built probe;    Membrane proteins;    Bicelles;    Bilayers;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

1H-15N heteronuclear dipolar coupling solid-state NMR experiments on lipid bilayer or bicelle samples are very useful for the structural studies of membrane proteins. However, to study these biological samples using solid-state NMR, a specific probe with high efficiency and high capability is required. In this paper, we describe the optimized design, construction, and efficiency of a 400 MHz wide-bore 1H-15N solid-state NMR probe with 5-mm solenoidal rf coil for high power, multi-pulse sequence experiments, such as 2D PISEMA or 2D SAMMY.

【 授权许可】

Unknown