Bulletin of the Korean chemical society | |
An NMR Study on the Phase Change of Lipid Membranes by an Antimicrobial Peptide, Protegrin-1 | |
Chul Kim1  | |
关键词: Antimicrobial peptide; Protegrin-1; Toroidal pore; 2H solid-state NMR; Phase change; | |
DOI : | |
学科分类:化学(综合) | |
来源: Korean Chemical Society | |
【 摘 要 】
Membrane disruption by an antimicrobial peptide, protegrin-1 (PG-1), was investigated by measuring the 2H solid-state nuclear magnetic resonance (SSNMR) spectra of 1-palmitoyl-d31-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC_ d31) in the mixture of PG-1 and POPC_d31 lipids deposited on thin cover-glass plates. The experimental line shapes of anisotropic 2H SSNMR spectra measured at various peptide-to-lipid (P/L) ratios were simulated reasonably by assuming the mosaic spread of bilayers containing pore structures or the coexistence of the mosaic spread of bilayers and a fast-tumbling isotropic phase. Within a few days of incubation in the hydration chamber, the pores were formed by the peptide in the POPC_d31 and POPC_d31/cholesterol membranes. However, the formation of the pores was not clear in the POPC_d31/1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylglycerol (POPG) membrane. Over a hundred days after hydration, a rapidly rotating isotropic phase increased in the POPC_d31 and the POPC_d31/cholesterol membranes with the higher P/L ratios, but no isotropic phase appeared in the POPC_d31/POPG membrane. Cholesterol added in the POPC bilayer acted as a stabilizer of the pore structure and suppressed the formation of a fast-tumbling isotropic phase.
【 授权许可】
Unknown
【 预 览 】
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RO201912010242063ZK.pdf | 521KB | download |