A New Approach for Thermodynamic Study on the Binding of Human Serum Albumin with Cerium Chloride

Bulletin of the Korean chemical society

G Rezaei Behbehani¹  A Divsalar¹  A A Saboury¹  M R Ganjali¹  F Faridbod¹ 

关键词: Human Serum Albumin;    Isothermal titration calorimetry;    Cerium (III) chloride.;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Thermodynamics of the interaction between Cerium (III) chloride, Ce3+, with Human Serum Albumin, HSA, was investigated at pH 7.0 and 27 oC in phosphate buffer by isothermal titration calorimetry. Our recently solvation model was used to reproduce the enthalpies of HSA interaction by Ce3+. The solvation parameters recovered from our new model, attributed to the structural change of HSA and its biological activity. The interaction of HSA with Ce3+ showed a set of two binding sites with negative cooperativity. Ce3+ interacts with multiple sites on HSA affecting its biochemical and biophysical properties.

【 授权许可】

Unknown