期刊论文详细信息
Bulletin of the Korean chemical society | |
An ELISA-on-a-Chip Biosensor System for Early Screening of Listeria monocytogenes in Contaminated Food Products | |
aSung Min Seo1  Hee Jung Lee1  Eun Gyoung Oh1  Joo Ho Kim1  Jin Woo Jeon1  Hong Sik Yu1  Se Hwan Paek1  Il Hoon Cho1  Soon Bum Shin1  | |
关键词: Food-borne microorganism; Early detection; Quantitative analysis; High sensitivity; Immunomagnetic separation; | |
DOI : | |
学科分类:化学(综合) | |
来源: Korean Chemical Society | |
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【 摘 要 】
An enzyme-linked immunosorbent assay (ELISA)-on-a-chip (EOC) biosensor combined with cell concentration technology based on immuno-magnetic separation (IMS) was investigated for use as a potential tool for early screening of Listeria monocytogenes (L. monocytogenes) in food products. The target analyte is a well-known pathogenic foodborne microorganism and outbreaks of the food poisoning typically occur due to contamination of normal food products. Thus, the aim of this study was to develop a rapid and reliable sensor that could be utilized on a daily basis to test food products for the presence of this pathogenic microorganism. The sensor was optimized to provide a high detection capability (e.g., 5.9 �? 103 cells/mL) and, to eventually minimize cultivation time. The cell density was condensed using IMS prior to analysis. Since the concentration rate of IMS was greater than 100-fold, this combination resulted in a detection limit of 54 cells/mL. The EOC-IMS coupled analytical system was then applied to a real sample test of fish intestines. The system was able to detect L. monocytogenes at a concentration of 2.4 CFU/g after pre-enrichment for 6 h from the onset of cell cultivation. This may allow us to monitor the target analyte at a concentration less than 1 CFU/g within a 9 h-cultivation provided a doubling time of 40 min is typically maintained. Based on this estimation, the EOC-IMS system can screen and detect the presence of this microorganism in food products almost within working hours.【 授权许可】
Unknown
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RO201912010241374ZK.pdf | 905KB | ![]() |