Determination of Epinephrine Using Sodium Iodate in Chemiluminescence

Bulletin of the Korean chemical society

H. B. Lim¹  J. S. Lee¹ 

关键词: Chemiluminescence;    Epinephrine;    Luminol;    Sodium iodate;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Epinephrine was determined using a lab-made chemiluminescence (CL) system with air pump. Luminolsodium IO₄^? chemiluminescence system was employed to produce the luminescence of epinephrine. In the reaction, epinephrine was oxidized to produce superoxide or singlet oxygen by periodate in alkaline solution, which enhanced CL of luminol. For optimization, various buffers, such as phosphate, borate, and tris, were studied in this experiment. Compared to NaOH, the phosphate and borate buffer showed better reproducibility with similar sensitivity. Small amount of sample, 22 レL, was required for a measurement. The limit of quantification for epinephrine was obtained to be ~10^?9 g/mL after optimization.

【 授权许可】

Unknown