Microfluidic Immunoassay Platform Using Antibody-immobilized Glass Beads and Its Application for Detection of Escherichia coli O157:H7

Bulletin of the Korean chemical society

Youn Sang Kim¹  Yoonsun Yang¹  Nae Yoon Lee¹  Sungsu Park¹ 

关键词: Microchamber;    Glass bead;    IgG immobilization;    Pathogenic bacteria;    Escherichia coli O157:H7;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

We developed a microfluidic immunoassay platform for the detection of various analytes such as bacterial pathogen by packing antibody-immobilized glass beads in spatially-isolated microchambers on a microfluidic device. Primary amines of antibody were covalently conjugated to carboxyl-terminated glass beads previously treated with aminosilane followed by glutaraldehyde. Through this covalent binding, up to 905 レg immunoglobulin G (IgG) per gram of glass beads was immobilized. For application, glass beads attaching antibody specific to Escherichia coli O157:H7, a foodborne pathogen, were packed into a microfluidic device and used for the detection of the serotype. This prototype immunoassay device can be used for the simultaneous detection of multiple analytes by sequentially packing different-sized glass beads attaching different antibody in discrete microchambers on a single microfluidic device.

【 授权许可】

Unknown