Effect of Metal Ions on the Degradation and Adsorption of Two Cellobiohydrolases on Microcrystalline Cellulose

Bulletin of the Korean chemical society

Young Hun Jang¹  Dong Won Kim¹  Chang Suk Kim¹  Nam-Soo Lee¹ 

关键词: Cellobiohydrolases;    Metal ion;    Adsorption;    Degradation;    Kinetics.;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

To test the metal ion effect, hydrolysis experiments for two cellobiohydrolases (CBH�? and CBH �?) from Trichoderma reesei have been carried out in the presence of 10 mM metal ions, such as Cu⁺⁺, Mn⁺⁺, Ca⁺⁺, Hg⁺⁺, Ba⁺⁺, Pb⁺⁺, and Cd⁺⁺. The addition of Mn⁺⁺, Ba⁺⁺, and Ca⁺⁺(10 mM) during the hydrolysis of Avicel PH 101 caused an increase in the total reducing sugar (TRS) for CBH �? by 142, 135, and 114 percent, respectively. Those for CBH �? increased by 177, 175, and 115 percent, respectively. The Mn⁺⁺ was the most stimulatory metal ion, whereas Hg⁺⁺ was the most inhibitory metal ion. The adsorption experiments were performed to investigate how the influence of Mn⁺⁺ and Hg⁺⁺ on the hydrolysis is related to the adsorption of cellobiohydrolases on cellulose. The increase in TRS during hydrolysis by adding Mn⁺⁺ caused an increase in adsorption affinity (K_ad) and tightness (ツH_a). While, the decrease of TRS during hydrolysis by adding Hg⁺⁺ caused a decrease in the adsorption affinity (K_ad) and tightness (ツH_a). These results indicate the changes in the tightness and affinity of adsorption by adding metal ions play a crucial role in the degradation of the microcrystalline cellulose.

【 授权许可】

Unknown