Renaturation of telomere-binding proteins after the fractionation by SDS-polyacrylamide gel electrophoresis

Plant, Soil and Environment

Rotková G.:¹ 

关键词: method;    renaturation;    telomere-binding proteins;    telomeres;   

DOI  :  

学科分类：农业科学（综合）

来源: Czech Academy of Agricultural Sciences

【 摘 要 】

A simple method for identification and characterization of telomere-binding proteins is described in this article. After Sodium Dodecyl Sulphate-Polyacrylamide gel electrophoresis (SDS-PAGE), proteins are eluted, renatured and used for retardation analysis with labelled oligonucleotides corresponding to human and plant of telomeric sequences. We show here that this method is efficient to recover sequence-specific DNA-binding abilities of putative telomere-binding proteins.

【 授权许可】

Unknown