Alleles controlling apple skin colour and incompatibility in new Czech apple varieties with different degrees of resistance against Venturia inaequalis CKE.

Plant, Soil and Environment

Vejl P.¹  Zoufalá J.¹  Milec Z.¹  Blažková H.¹  Blažek J.¹  Melounová M.¹  Sedlák P.¹ 

关键词: Malus;    PCR;    Vf gene;    incompatibility;    S-allele;    skin colour;    A-allele;   

DOI  :  

学科分类：农业科学（综合）

来源: Czech Academy of Agricultural Sciences

【 摘 要 】

The skin colour of 21 varieties was assessed on a molecular level. Four varieties had yellow skin colour with the genotype constitution a1a1, a1a2 or a2a2. Seventeen varieties had dominant red colour. Homozygous A1A1 constitution was present in 7 varieties, and heterozygous constitution A1a1 or A1a2 was found in the rest of the assessed varieties. The S-alleles controlled the incompatibility system of the pistil. Their detection is possible by means of the PCR method on the basis of allele specific primers. Six S-alleles (S1, S2, S3, S5, S7 and S9) were studied in the same collection of 21 diploid Czech varieties. This paper brings new findings on S-allele characterization, because the Czech varieties have not yet been studied on a molecular level. Both types of S-allele were found in 12 varieties. Only one type of S-allele was described in 9 varieties. Simultaneously, the presence of the Vf gene was screened in the collection of 21 Czech apple varieties. Ten varieties with a field resistance against the scab had a heterozygous constitution of the Vf gene. All 11 susceptible varieties were recessive homozygous vfvf.

【 授权许可】

Unknown