Plant, Soil and Environment | |
Alleles controlling apple skin colour and incompatibility in new Czech apple varieties with different degrees of resistance against Venturia inaequalis CKE. | |
Vejl P.1  Zoufalá J.1  Milec Z.1  Blažková H.1  Blažek J.1  Melounová M.1  Sedlák P.1  | |
关键词: Malus; PCR; Vf gene; incompatibility; S-allele; skin colour; A-allele; | |
DOI : | |
学科分类:农业科学(综合) | |
来源: Czech Academy of Agricultural Sciences | |
【 摘 要 】
The skin colour of 21 varieties was assessed on a molecular level. Four varieties had yellow skin colour with the genotype constitution a1a1, a1a2 or a2a2. Seventeen varieties had dominant red colour. Homozygous A1A1 constitution was present in 7 varieties, and heterozygous constitution A1a1 or A1a2 was found in the rest of the assessed varieties. The S-alleles controlled the incompatibility system of the pistil. Their detection is possible by means of the PCR method on the basis of allele specific primers. Six S-alleles (S1, S2, S3, S5, S7 and S9) were studied in the same collection of 21 diploid Czech varieties. This paper brings new findings on S-allele characterization, because the Czech varieties have not yet been studied on a molecular level. Both types of S-allele were found in 12 varieties. Only one type of S-allele was described in 9 varieties. Simultaneously, the presence of the Vf gene was screened in the collection of 21 Czech apple varieties. Ten varieties with a field resistance against the scab had a heterozygous constitution of the Vf gene. All 11 susceptible varieties were recessive homozygous vfvf.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912010232796ZK.pdf | 274KB | download |