Identification of sex in F1 progenies of hop (Humulus lupulus L.) by molecular marker

Plant, Soil and Environment

Skupinová S.¹  Vejl P.¹  Nesvadba V.:¹  Patzak J.¹ 

关键词: PCR;    marker-assisted selection (MAS);   

DOI  :  

学科分类：农业科学（综合）

来源: Czech Academy of Agricultural Sciences

【 摘 要 】

Sex identification for hop (Humulus lupulus L.), which is a dioecious plant, is very important for breeding process. The use of molecular methods for marker-assisted selection (MAS) provides rapid and reliable identification of sex in F1 progenies. In the first, we proved the use of specific PCR molecular marker for evaluation of sex in selected plants. This marker was not amplified in 3 from 35 male plants. In the next, we successfully analysed 770 genotypes of F1 progenies of three crossings. The amplified differences were found in 4 male plants of crossing Sm01 H28, 8 male plants of crossing Sm01 H29 and 5 female plants of crossing Sm00 H20. Statistic analysis confirmed that sex ratios of all F1 progenies were significantly coincident. Obtained results confirmed that this specific PCR marker successfully identified sex of juvenile hop plants in F1 progenies and effectively improved breeding process.Keywords:PCR; marker-assisted selection (MAS)download PDFImpact factor (Web of Science �? Thomson Reuters)2016: 1.2255-Year Impact Factor: 1.463SJR (SCImago Journal Rank �? SCOPUS)2016: 0.616 �? Q2 (Soil Science)Similarity CheckAll the submitted manuscripts are checked by the CrossRef Similarity Check.Abstracts are comprised in the databasesISI Web of KnowledgeSM, Web of Science®, Science Citation Index Expanded®, Agricola, Agrindex of AGRIS/FAO databáze, AGRIS International, CAB Abstracts, Current Contents®/Agriculture, Biology and Environmental Sciences, Czech Agricultural and Food Bibliography, Food Science and Technology Abstracts, SCOPUS, TOXLINE Plus, DOAJ (Directory of Open Access Journals), Google Scholar, CNKILicence termsAll content is made freely available for non-commercial purposes, users are allowed to copy and redistribute the material, transform, and build upon the material as long as they cite the source.Open Access PolicyThis journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge.ContactMgr. Kateřina SoučkováExecutive Editorphone: + 420 606 402 207e-mail: pseAddressPlant, Soil and EnvironmentCzech Academy of Agricultural SciencesSlezská 7, 120 00 Praha 2,Czech Republic© 2017 Czech Academy of Agricultural Sciences

【 授权许可】

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