HS-GC Method Validation for Determination of Residual Solvents In Herbal Extracts

RGUHS Journal of Pharmaceutical Sciences

B. Murali²  Datendra Nath Tripathi¹  Monica Kachroo¹ 

[1] Al- Ameen College of Pharmacy, Near Lalbagh Main Gate, Bangalore-560027 Al- Ameen College of Pharmacy, Near Lalbagh Main Gate, Bangalore-560027 Al- Ameen College of Pharmacy, Near Lalbagh Main Gate, Bangalore-560027;Natural Remedies Private limited, Veersandra Indl. Area, Hosur Road, Bangalore Natural Remedies Private limited, Veersandra Indl. Area, Hosur Road, Bangalore Natural Remedies Private limited, Veersandra Indl. Area, Hosur Road, Bangalore

关键词: Herbal extract;    residual solvents;    gas chromatography;    Flame ionization detector;   

DOI  :  10.5530/rjps.2012.3.10

学科分类：药学、药理学、毒理学（综合）

来源: Rajiv Gandhi University of Health Sciences

【 摘 要 】

Residual solvent in herbal extracts were monitored using head space gas chromatograph (HS-GC) with fl ame ionization detector (FID) and headspace AOC 5000 auto sampler. The separation of the residual solvents was achieved on a Zebron (ZB-624) column (30 m X 0.25 mm i.d. X 0.25 μm coating thickness) using GC 2010 Shimadzu, with nitrogen as a carrier gas in the split mode by headspace method. Fast separation of 7 commonly used solvents (methanol, ethanol, acetone, n-hexane, ethyl acetate, ethylene dichloride and toluene) was achieved in a single analysis of 9.19 min run time with 1.95 ml/min fl ow rate. The oven and detector temperature was 34°C and 255°C respectively using N,N’-Dimethylformamide(DMF) as a diluent. The method was successfully validated for linearity, specifi city, precision, accuracy, and range with good recoveries. The linear range for standard mix solvent was 10 to 200 ppm.

【 授权许可】

Unknown