RGUHS Journal of Pharmaceutical Sciences | |
HS-GC Method Validation for Determination of Residual Solvents In Herbal Extracts | |
B. Murali2  Datendra Nath Tripathi1  Monica Kachroo1  | |
[1] Al- Ameen College of Pharmacy, Near Lalbagh Main Gate, Bangalore-560027 Al- Ameen College of Pharmacy, Near Lalbagh Main Gate, Bangalore-560027 Al- Ameen College of Pharmacy, Near Lalbagh Main Gate, Bangalore-560027;Natural Remedies Private limited, Veersandra Indl. Area, Hosur Road, Bangalore Natural Remedies Private limited, Veersandra Indl. Area, Hosur Road, Bangalore Natural Remedies Private limited, Veersandra Indl. Area, Hosur Road, Bangalore | |
关键词: Herbal extract; residual solvents; gas chromatography; Flame ionization detector; | |
DOI : 10.5530/rjps.2012.3.10 | |
学科分类:药学、药理学、毒理学(综合) | |
来源: Rajiv Gandhi University of Health Sciences | |
【 摘 要 】
Residual solvent in herbal extracts were monitored using head space gas chromatograph (HS-GC) with fl ame ionization detector (FID) and headspace AOC 5000 auto sampler. The separation of the residual solvents was achieved on a Zebron (ZB-624) column (30 m X 0.25 mm i.d. X 0.25 μm coating thickness) using GC 2010 Shimadzu, with nitrogen as a carrier gas in the split mode by headspace method. Fast separation of 7 commonly used solvents (methanol, ethanol, acetone, n-hexane, ethyl acetate, ethylene dichloride and toluene) was achieved in a single analysis of 9.19 min run time with 1.95 ml/min fl ow rate. The oven and detector temperature was 34°C and 255°C respectively using N,N’-Dimethylformamide(DMF) as a diluent. The method was successfully validated for linearity, specifi city, precision, accuracy, and range with good recoveries. The linear range for standard mix solvent was 10 to 200 ppm.
【 授权许可】
Unknown
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