Evidence for Population-Specific Positive Selection on Immune Genes of Anopheles gambiae

G3: Genes, Genomes, Genetics

Inge Holm²  Michelle M. Riehle⁴  N’Fale Sagnon³  Karin Eiglmeier²  Kenneth D. Vernick²  Thierry Garnier²  Wamdaogo M. Guelbeogo³  Jacob E. Crawford¹  Brian P. Lazzaro¹  Emmanuel Bischoff²  Awa Gneme³ 

[1] Department of Entomology, Cornell University, Ithaca, New York 14853Department of Entomology, Cornell University, Ithaca, New York 14853Department of Entomology, Cornell University, Ithaca, New York 14853;Unit of Insect Vector Genetics and Genomics, Department of Parasitology and Mycology, Institut Pasteur, F-75015 Paris, FranceCentre National de la Recherche Scientifique (CNRS), URA 3012, F-75015 Paris, FranceUnit of Insect Vector Genetics and Genomics, Department of Parasitology and Mycology, Institut Pasteur, F-75015 Paris, FranceUnit of Insect Vector Genetics and Genomics, Department of Parasitology and Mycology, Institut Pasteur, F-75015 Paris, FranceCentre National de la Recherche Scientifique (CNRS), URA 3012, F-75015 Paris, FranceCentre National de la Recherche Scientifique (CNRS), URA 3012, F-75015 Paris, FranceUnit of Insect Vector Genetics and Genomics, Department of Parasitology and Mycology, Institut Pasteur, F-75015 Paris, FranceCentre National de la Recherche Scientifique (CNRS), URA 3012, F-75015 Paris, France;Centre National de Recherche et de Formation sur le Paludisme, 01 BP 2208 Ouagadougou, Burkina FasoCentre National de Recherche et de Formation sur le Paludisme, 01 BP 2208 Ouagadougou, Burkina FasoCentre National de Recherche et de Formation sur le Paludisme, 01 BP 2208 Ouagadougou, Burkina Faso;Microbial and Plant Genomics Institute, Department of Microbiology, University of Minnesota, Saint Paul, Minnesota 55108Microbial and Plant Genomics Institute, Department of Microbiology, University of Minnesota, Saint Paul, Minnesota 55108Microbial and Plant Genomics Institute, Department of Microbiology, University of Minnesota, Saint Paul, Minnesota 55108

关键词: Anopheles gambiae;    positive selection;    host−pathogen interaction;    immune gene;    Plasmodium falciparum;   

DOI  :  10.1534/g3.112.004473

学科分类：生物科学（综合）

来源: Genetics Society of America

【 摘 要 】

Host-pathogen interactions can be powerful drivers of adaptive evolution, shaping the patterns of molecular variation at the genes involved. In this study, we sequenced alleles from 28 immune-related loci in wild samples of multiple genetic subpopulations of the African malaria mosquito Anopheles gambiae, obtaining unprecedented sample sizes and providing the first opportunity to contrast patterns of molecular evolution at immune-related loci in the recently discovered GOUNDRY population to those of the indoor-resting M and S molecular forms. In contrast to previous studies that focused on immune genes identified in laboratory studies, we centered our analysis on genes that fall within a quantitative trait locus associated with resistance to Plasmodium falciparum in natural populations of A. gambiae. Analyses of haplotypic and genetic diversity at these 28 loci revealed striking differences among populations in levels of genetic diversity and allele frequencies in coding sequence. Putative signals of positive selection were identified at 11 loci, but only one was shared by two subgroups of A. gambiae. Striking patterns of linkage disequilibrium were observed at several loci. We discuss these results with respect to ecological differences among these strata as well as potential implications for disease transmission.

【 授权许可】

Unknown