PUF-8, a Pumilio Homolog, Inhibits the Proliferative Fate in the Caenorhabditis elegans Germline

G3: Genes, Genomes, Genetics

Hilary Racher¹  Dave Hansen¹ 

[1] Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada, T2N 1N4Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada, T2N 1N4Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada, T2N 1N4

关键词: puf-8;    Pumilio;    PUF;    germline stem cells;    proliferative fate;    Notch signaling;   

DOI  :  10.1534/g3.112.003350

学科分类：生物科学（综合）

来源: Genetics Society of America

【 摘 要 】

Stem cell populations are maintained by keeping a balance between self-renewal (proliferation) and differentiation of dividing stem cells. Within the Caenorhabditis elegans germline, the key regulator maintaining this balance is the canonical Notch signaling pathway, with GLP-1/Notch activity promoting the proliferative fate. We identified the Pumilio homolog, PUF-8, as an inhibitor of the proliferative fate of stem cells in the C. elegans germline. puf-8(0) strongly enhances overproliferation of glp-1(gf) mutants and partially suppresses underproliferation of a weak glp-1(lf) mutant. The germline tumor that is formed in a puf-8(0); glp-1(gf) double mutant is due to a failure of germ cells to enter meiotic prophase. puf-8 likely inhibits the proliferative fate through negatively regulating GLP-1/Notch signaling or by functioning parallel to it.

【 授权许可】

Unknown