G3: Genes, Genomes, Genetics | |
Double-Strand Break Repair and Holliday Junction Processing Are Required for Chromosome Processing in Stationary-Phase Escherichia coli Cells | |
Ashley B. Williams1  Kyle M. Hetrick2  Patricia L. Foster2  | |
[1] Molecular and Computational Biology, University of Southern California, Los Angeles, California 90089Molecular and Computational Biology, University of Southern California, Los Angeles, California 90089Molecular and Computational Biology, University of Southern California, Los Angeles, California 90089;Department of Biology, Indiana University, Bloomington, Indiana 47405Department of Biology, Indiana University, Bloomington, Indiana 47405Department of Biology, Indiana University, Bloomington, Indiana 47405 | |
关键词: DNA repair; stationary phase; homologous recombination; genome stability; double-strand break repair; | |
DOI : 10.1534/g3.111.001057 | |
学科分类:生物科学(综合) | |
来源: Genetics Society of America | |
【 摘 要 】
As nutrients are depleted and cell division ceases in batch cultures of bacteria, active processes are required to ensure that each cell has a complete copy of its genome. How chromosome number is manipulated and maintained in nondividing bacterial cells is not fully understood. Using flow cytometric analysis of cells from different growth phases, we show that the Holliday junction–processing enzymes RuvABC and RecG, as well as RecBCD, the enzyme complex that initiates DNA double-strand break repair, are required to establish the normal distribution of fluorescent peaks, which is commonly accepted to reflect the distribution of chromosome numbers. Our results reveal that these proteins are required for the proper processing of chromosomes in stationary phase.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912010200313ZK.pdf | 1859KB | download |