Fluorescence In Situ Hybridization–Based Karyotyping of Soybean Translocation Lines

G3: Genes, Genomes, Genetics

Yaya Cui²  Gary Stacey²  Reid G. Palmer³  James A. Birchler¹  Allison L. Pappas³  Seth D. Findley² 

[1] Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211;National Center for Soybean Biotechnology, Division of Plant Sciences, University of Missouri, Columbia, Missouri 65211National Center for Soybean Biotechnology, Division of Plant Sciences, University of Missouri, Columbia, Missouri 65211National Center for Soybean Biotechnology, Division of Plant Sciences, University of Missouri, Columbia, Missouri 65211;USDA-ARS, Iowa State University, Ames, Iowa 50011USDA-ARS, Iowa State University, Ames, Iowa 50011USDA-ARS, Iowa State University, Ames, Iowa 50011

关键词: soybean;    chromosome translocation;    FISH;    karyotype;    cytogenetics;   

DOI  :  10.1534/g3.111.000034

学科分类：生物科学（综合）

来源: Genetics Society of America

【 摘 要 】

Soybean (Glycine max [L.] Merr.) is a major crop species and, therefore, a major target of genomic and genetic research. However, in contrast to other plant species, relatively few chromosomal aberrations have been identified and characterized in soybean. This is due in part to the difficulty of cytogenetic analysis of its small, morphologically homogeneous chromosomes. The recent development of a fluorescence in situ hybridization –based karyotyping system for soybean has enabled our characterization of most of the chromosomal translocation lines identified to date. Utilizing genetic data from existing translocation studies in soybean, we identified the chromosomes and approximate breakpoints involved in five translocation lines.

【 授权许可】

Unknown