Translational Genomics in Legumes Allowed Placing In Silico 5460 Unigenes on the Pea Functional Map and Identified Candidate Genes in Pisum sativum L.

G3: Genes, Genomes, Genetics

Grégoire Aubert¹  Judith Burstin¹  Céline Rond¹  Pascal Marget¹  Vincent Savois¹  Michael Bourgeois¹  Jérome Salse²  Amandine Bordat¹  Florent Murat²  Hervé Houtin¹  Jean Potier¹  Marie Nicolas¹  Aurélie Chauveau¹ 

[1] INRA, UMRLEG, 21065 Dijon Cedex, FranceINRA, UMRLEG, 21065 Dijon Cedex, FranceINRA, UMRLEG, 21065 Dijon Cedex, France;INRA, UMR1095, 63100 Clermont-Ferrand, FranceINRA, UMR1095, 63100 Clermont-Ferrand, FranceINRA, UMR1095, 63100 Clermont-Ferrand, France

关键词: Pisum sativum;    functional consensus map;    synteny;    model legume species;    translational genomics;   

DOI  :  10.1534/g3.111.000349

学科分类：生物科学（综合）

来源: Genetics Society of America

【 摘 要 】

To identify genes involved in phenotypic traits, translational genomics from highly characterized model plants to poorly characterized crop plants provides a valuable source of markers to saturate a zone of interest as well as functionally characterized candidate genes. In this paper, an integrated view of the pea genetic map was developed. A series of gene markers were mapped and their best reciprocal homologs were identified on M. truncatula, L. japonicus, soybean, and poplar pseudomolecules. Based on the syntenic relationships uncovered between pea and M. truncatula, 5460 pea Unigenes were tentatively placed on the consensus map. A new bioinformatics tool, http://www.thelegumeportal.net/pea_mtr_translational_toolkit, was developed that allows, for any gene sequence, to search its putative position on the pea consensus map and hence to search for candidate genes among neighboring Unigenes. As an example, a promising candidate gene for the hypernodulation mutation nod3 in pea was proposed based on the map position of the likely homolog of Pub1, a M. truncatula gene involved in nodulation regulation. A broader view of pea genome evolution was obtained by revealing syntenic relationships between pea and sequenced genomes. Blocks of synteny were identified which gave new insights into the evolution of chromosome structure in Papillionoids and Eudicots. The power of the translational genomics approach was underlined.

【 授权许可】

Unknown