Imaging DNA Damage Allows Detection of Preneoplasia in the BALB-neuT Model of Breast Cancer

Journal of Nuclear Medicine

Jean-Baptiste Cazier¹  Federica Cavallo¹  Manuela Iezzi¹  Bart Cornelissen¹  Katherine A. Vallis¹  Sarah Able¹  James Knight¹  Sean Smart¹  Veerle Kersemans¹  P. Danny Allen¹  Christiana Kartsonaki¹  Ruth Muschel¹ 

关键词: γH2AX;    molecular imaging;    BALB-NeuT;    breast cancer;    early diagnosis;   

DOI  :  10.2967/jnumed.114.142083

学科分类：医学（综合）

来源: Society of Nuclear Medicine

【 摘 要 】

A prominent feature of many human cancers is oncogene-driven activation of the DNA damage response (DDR) during early tumorigenesis. It has been shown previously that noninvasive imaging of the phosphorylated histone H2A variant H2AX, γH2AX, a DNA damage signaling protein, is possible using 111In-labeled anti-γH2AX antibody conjugated to the cell-penetrating peptide transactivator of transcription (TAT). The purpose of this study was to investigate whether 111In-anti-γH2AX-TAT detects the DDR during mammary oncogenesis in BALB-neuT mice. Methods: Mammary fat pads from BALB-neuT and wild-type mice (age, 40–106 d) were immunostained for γH2AX. 111In-anti-γH2AX-TAT or a control probe was administered intravenously to BALB-neuT mice. SPECT was performed weekly and compared with tumor detection using palpation and dynamic contrast-enhanced MR imaging. Results: γH2AX expression was elevated in hyperplastic lesions in the mammary fat pads of BALB-neuT mice aged 76–106 d, compared with normal fat pads from younger mice and carcinomas from older mice (13.5 ± 1.2 γH2AX foci/cell vs. 5.2 ± 1.5 [P < 0.05] and 3.4 ± 1.1 [P < 0.001], respectively). Serial SPECT imaging revealed a 2.5-fold increase in 111In-anti-γH2AX-TAT accumulation in the mammary fat pads of mice aged 76–106 d, compared with control probe (P = 0.01). The median time to detection of neoplastic lesions by 111In-anti-γH2AX-TAT (defined as >5% injected dose per gram of tissue) was 96 d, compared with 120 and 131 d for dynamic contrast-enhanced MR imaging and palpation, respectively (P < 0.001). Conclusion: DDR imaging using 111In-anti-γH2AX-TAT identified mammary tumors significantly earlier than MR imaging. Imaging the DDR holds promise for the detection of preneoplasia and as a technique for screening cancer-prone individuals.

【 授权许可】

Unknown