Fast Metabolic Response to Drug Intervention Through Analysis on a Miniaturized, Highly Integrated Molecular Imaging System

Journal of Nuclear Medicine

Michael E. Phelps¹  Yuchao Gu¹  Dean O. Campbell¹  Daniel Braas¹  David Nathanson¹  Arion F. Chatziioannou¹  Heather Christofk¹  Jun Wang¹  Kiwook Hwang¹  Caius Radu¹  Alex Dooraghi¹  James R. Heath¹  Paul Mischel¹  Troy Sandberg¹ 

关键词: microfluidics;    molecular imaging;    radiopharmaceuticals;    radioassay;   

DOI  :  10.2967/jnumed.112.118497

学科分类：医学（综合）

来源: Society of Nuclear Medicine

【 摘 要 】

We report on a radiopharmaceutical imaging platform designed to capture the kinetics of cellular responses to drugs. Methods: A portable in vitro molecular imaging system comprising a microchip and a β-particle imaging camera permitted routine cell-based radioassays of small numbers of either suspended or adherent cells. We investigated the kinetics of responses of model lymphoma and glioblastoma cancer cell lines to 18F-FDG uptake after drug exposure. Those responses were correlated with kinetic changes in the cell cycle or with changes in receptor tyrosine kinase signaling. Results: The platform enabled direct radioassays of multiple cell types and yielded results comparable to those from conventional approaches; however, the platform used smaller sample sizes, permitted a higher level of quantitation, and did not require cell lysis. Conclusion: The kinetic analysis enabled by the platform provided a rapid (∼1 h) drug screening assay.

【 授权许可】

Unknown