Reduced Iodide Transport (Stunning) and DNA Synthesis in Thyrocytes Exposed to Low Absorbed Doses from 131I In Vitro

Journal of Nuclear Medicine

Madeleine M. Nordén¹  Charlotta Lundh¹  Eva Forssell-Aronsson¹  Mikael Nilsson¹ 

关键词: thyroid;    radioiodine;    ¹³¹I;    stunning;    iodide transport;    dosimetry;   

DOI  :  

学科分类：医学（综合）

来源: Society of Nuclear Medicine

【 摘 要 】

Thyroid stunning refers to reduced uptake of 131I in the thyroid tissue (or tumor) during radioiodine (131I) therapy compared with the uptake measured after the previous administration of 131I for diagnostic purposes. The phenomenon is clinically important, as it can potentially lead to the undertreatment of thyroid cancer or to unnecessarily high absorbed doses in critical organs. Previous clinical and experimental studies indicated that thyroid stunning is absorbed dose dependent. The aim of this study was to investigate the effects of 131I irradiation on 125I− transport and cell proliferation at low absorbed doses in vitro. Methods: Primary cultured porcine thyroid cells were grown to form a confluent monolayer of epithelial cells on a filter in a bicameral culture system. The cells were continuously irradiated with 131I in the culture medium for 48 h to obtain 0.0015–1.5 Gy. At 3 d after irradiation was stopped, the transepithelial iodide transport capacity was evaluated by measuring 125I− transport from the basal chamber compartment to the apical chamber compartment. The effect of 131I irradiation on DNA synthesis was estimated by pulse labeling with 3H-thymidine of both subconfluent and confluent cells irradiated with up to 9 Gy. Total DNA content was measured to quantify cell numbers. Results: A statistically significant reduction in 125I− transport was seen at absorbed doses of ≥0.15 Gy, with a 50% reduction at 1.5 Gy, compared with the results observed for nonirradiated control cells. 3H-Thymidine incorporation was already statistically significantly reduced at absorbed doses of 0.01–0.1 Gy, but 0.15–0.3 Gy did not affect DNA synthesis. However, absorbed doses of ≥1 Gy again resulted in reduced DNA synthesis. A 50% reduction was obtained at 4 Gy. Total DNA measurements revealed a statistically significant reduction in cell numbers at 8 Gy. Conclusion: The lowest absorbed dose from 131I that reduced iodide transport was 0.15 Gy. Because stunning was found at low absorbed doses, it might occur for 131I treatment not only of thyroid cancer but also of thyrotoxicosis. On the basis of differences in dose responses, radiation-induced thyroid stunning and cell cycle arrest may be independent phenomena.

【 授权许可】

Unknown