Stunning of Iodide Transport by 131I Irradiation in Cultured Thyroid Epithelial Cells

Journal of Nuclear Medicine

Svante Jansson¹  Ulrika Lindencrona¹  Jakob Himmelman¹  Per Postgård¹  Eva Forssell-Aronsson¹  Gertrud Berg¹  Dennis Wiberg¹  Ernst Nyström¹  Mikael Nilsson¹  Nirmal Bhogal¹ 

关键词: radioiodide;    stunning;    thyroid;    ¹³¹I;   

DOI  :  

学科分类：医学（综合）

来源: Society of Nuclear Medicine

【 摘 要 】

The existence of thyroid stunning (i.e., inhibited thyroidal iodide uptake after administration of diagnostic amounts of 131I) is controversial and is currently a subject of debate. To our knowledge, the stunning phenomenon has not been investigated previously in vitro. Methods: Growth-arrested porcine thyroid cells that formed a tight and polarized monolayer in a bicameral chamber were irradiated with 3–80 Gy 131I present in the surrounding culture medium for 48 h. The iodide transport capacity after irradiation was evaluated 3 d later by measuring the transepithelial (basal to apical) flux of trace amounts of 125I. Results: The basal-to-apical 125I transport decreased with increasing absorbed dose acquired from 131I; a nearly 50% reduction was observed already at 3 Gy. Stable iodide at the same molarity as 131I (10−8 mol/L) had no effect on the 125I transport. Cell number and epithelial integrity were not affected by irradiation. Conclusion: Stunning of iodide transport is detected after 131I irradiation of cultured thyroid cells. The degree of inhibition of transport is dependent on the absorbed dose.

【 授权许可】

Unknown