Simple Analytic Method of 11C-Flumazenil Metabolite in Blood

Journal of Nuclear Medicine

Koichi Ishizu¹  Junji Konishi¹  Masafumi Ihara¹  Yasuhiro Magata¹  Hideo Saji¹  Sadahiko Nishizawa¹  Haruhiro Kitano¹  Takahiro Mukai¹ 

关键词: positron;    benzodiazemine receptor;    flumazenil;    input function;   

DOI  :  

学科分类：医学（综合）

来源: Society of Nuclear Medicine

【 摘 要 】

11C-Flumazenil (11C-FMZ) is useful to estimate central benzodiazepine receptors by PET. The binding potential (BP) can be calculated with dynamic PET and continual blood sampling. Because conventional metabolite analysis of plasma samples is complicated and time consuming, a simple method is required to obtain an input function. In this article, a whole blood solvent extraction method was evaluated using data of 13 subjects. Methods: The plasma solvent extraction method was estimated in comparison with the thin-layer chromatography (TLC) method. Then, the whole blood solvent extraction method was evaluated in comparison with the plasma solvent extraction method. Results: Metabolite data analyzed by the plasma extraction method were well correlated with those by the TLC method (r = 0.99). The BP was calculated using both the whole blood extraction data and the plasma extraction data. No difference was observed in all brain regions. Conclusion: This simple whole blood solvent extraction method can be applied to clinical BP estimation using 11C-FMZ.

【 授权许可】

Unknown