Phosphotyrosine profiling of curcumin-induced signaling

Clinical Proteomics

Harsha Gowda⁸  Hitendra S. Solanki²  Bipin Nair⁹  Aafaque Ahmad Khan²  Gajanan Sathe³  Arun H. Patil²  Sandip Chavan³  T. S. Keshava Prasad⁴  Premendu Prakash Mathur⁷  Aditi Chatterjee⁸  Sneha M. Pinto⁸  Raja Sekhar Nirujogi¹  Santosh Renuse⁵  Vishalakshi Nanjappa⁵  Nazia Syed⁶ 

[1] Institute of Bioinformatics, Bangalore, IndiaCentre of Excellence in Bioinformatics, School of Life Sciences, Pondicherry University, Puducherry, IndiaInstitute of Bioinformatics, Bangalore, IndiaInstitute of Bioinformatics, Bangalore, IndiaCentre of Excellence in Bioinformatics, School of Life Sciences, Pondicherry University, Puducherry, IndiaCentre of Excellence in Bioinformatics, School of Life Sciences, Pondicherry University, Puducherry, IndiaInstitute of Bioinformatics, Bangalore, IndiaCentre of Excellence in Bioinformatics, School of Life Sciences, Pondicherry University, Puducherry, India;Institute of Bioinformatics, Bangalore, IndiaSchool of Biotechnology, KIIT University, Bhubaneswar, IndiaInstitute of Bioinformatics, Bangalore, IndiaInstitute of Bioinformatics, Bangalore, IndiaSchool of Biotechnology, KIIT University, Bhubaneswar, IndiaSchool of Biotechnology, KIIT University, Bhubaneswar, IndiaInstitute of Bioinformatics, Bangalore, IndiaSchool of Biotechnology, KIIT University, Bhubaneswar, India;Institute of Bioinformatics, Bangalore, IndiaManipal University, Manipal, IndiaInstitute of Bioinformatics, Bangalore, IndiaInstitute of Bioinformatics, Bangalore, IndiaManipal University, Manipal, IndiaManipal University, Manipal, IndiaInstitute of Bioinformatics, Bangalore, IndiaManipal University, Manipal, India;Institute of Bioinformatics, Bangalore, IndiaYU-IOB Center for Systems Biology and Molecular Medicine, Yenepoya University, Mangalore, IndiaNIMHANS-IOB Proteomics and Bioinformatics Laboratory, Neurobiology Research Centre, National Institute of Mental Health and Neurosciences, Bangalore, IndiaInstitute of Bioinformatics, Bangalore, IndiaInstitute of Bioinformatics, Bangalore, IndiaYU-IOB Center for Systems Biology and Molecular Medicine, Yenepoya University, Mangalore, IndiaNIMHANS-IOB Proteomics and Bioinformatics Laboratory, Neurobiology Research Centre, National Institute of Mental Health and Neurosciences, Bangalore, IndiaYU-IOB Center for Systems Biology and Molecular Medicine, Yenepoya University, Mangalore, IndiaInstitute of Bioinformatics, Bangalore, IndiaYU-IOB Center for Systems Biology and Molecular Medicine, Yenepoya University, Mangalore, IndiaNIMHANS-IOB Proteomics and Bioinformatics Laboratory, Neurobiology Research Centre, National Institute of Mental Health and Neurosciences, Bangalore, IndiaNIMHANS-IOB Proteomics and Bioinformatics Laboratory, Neurobiology Research Centre, National Institute of Mental Health and Neurosciences, Bangalore, IndiaInstitute of Bioinformatics, Bangalore, IndiaYU-IOB Center for Systems Biology and Molecular Medicine, Yenepoya University, Mangalore, IndiaNIMHANS-IOB Proteomics and Bioinformatics Laboratory, Neurobiology Research Centre, National Institute of Mental Health and Neurosciences, Bangalore, India;Institute of Bioinformatics, Bangalore, IndiaAmrita School of Biotechnology, Amrita University, Kollam, IndiaInstitute of Bioinformatics, Bangalore, IndiaInstitute of Bioinformatics, Bangalore, IndiaAmrita School of Biotechnology, Amrita University, Kollam, IndiaAmrita School of Biotechnology, Amrita University, Kollam, IndiaInstitute of Bioinformatics, Bangalore, IndiaAmrita School of Biotechnology, Amrita University, Kollam, India;Institute of Bioinformatics, Bangalore, IndiaDepartment of Biochemistry and Molecular Biology, Pondicherry University, Puducherry, IndiaInstitute of Bioinformatics, Bangalore, IndiaInstitute of Bioinformatics, Bangalore, IndiaDepartment of Biochemistry and Molecular Biology, Pondicherry University, Puducherry, IndiaDepartment of Biochemistry and Molecular Biology, Pondicherry University, Puducherry, IndiaInstitute of Bioinformatics, Bangalore, IndiaDepartment of Biochemistry and Molecular Biology, Pondicherry University, Puducherry, India;School of Biotechnology, KIIT University, Bhubaneswar, IndiaSchool of Biotechnology, KIIT University, Bhubaneswar, IndiaSchool of Biotechnology, KIIT University, Bhubaneswar, India;Institute of Bioinformatics, Bangalore, IndiaYU-IOB Center for Systems Biology and Molecular Medicine, Yenepoya University, Mangalore, IndiaInstitute of Bioinformatics, Bangalore, IndiaInstitute of Bioinformatics, Bangalore, IndiaYU-IOB Center for Systems Biology and Molecular Medicine, Yenepoya University, Mangalore, IndiaYU-IOB Center for Systems Biology and Molecular Medicine, Yenepoya University, Mangalore, IndiaInstitute of Bioinformatics, Bangalore, IndiaYU-IOB Center for Systems Biology and Molecular Medicine, Yenepoya University, Mangalore, India;Amrita School of Biotechnology, Amrita University, Kollam, IndiaAmrita School of Biotechnology, Amrita University, Kollam, IndiaAmrita School of Biotechnology, Amrita University, Kollam, India

关键词: Oral cancer;    Phosphoproteomics;    In vivo labeling;    Curcumin;   

DOI  :  10.1186/s12014-016-9114-0

来源: Humana Press Inc

【 摘 要 】

Abstract

Background

Curcumin, derived from the rhizome Curcuma longa, is a natural anti-cancer agent and has been shown to inhibit proliferation and survival of tumor cells. Although the anti-cancer effects of curcumin are well established, detailed understanding of the signaling pathways altered by curcumin is still lacking. In this study, we carried out SILAC-based quantitative proteomic analysis of a HNSCC cell line (CAL 27) to investigate tyrosine signaling in response to curcumin.

Results

Using high resolution Orbitrap Fusion Tribrid Fourier transform mass spectrometer, we identified 627 phosphotyrosine sites mapping to 359 proteins. We observed alterations in the level of phosphorylation of 304 sites corresponding to 197 proteins upon curcumin treatment. We report here for the first time, curcumin-induced alterations in the phosphorylation of several kinases including TNK2, FRK, AXL, MAPK12 and phosphatases such as PTPN6, PTPRK, and INPPL1 among others. Pathway analysis revealed that the proteins differentially phosphorylated in response to curcumin are known to be involved in focal adhesion kinase signaling and actin cytoskeleton reorganization.

Conclusions

The study indicates that curcumin may regulate cellular processes such as proliferation and migration through perturbation of the focal adhesion kinase pathway. This is the first quantitative phosphoproteomics-based study demonstrating the signaling events that are altered in response to curcumin. Considering the importance of curcumin as an anti-cancer agent, this study will significantly improve the current knowledge of curcumin-mediated signaling in cancer.

【 授权许可】

Unknown