Urinary Peptidomic Analysis Identifies Potential Biomarkers for Acute Rejection of Renal Transplantation

Clinical Proteomics

Xuefeng B. Ling¹  Tara K. Sigdel³  Ken H. Lau¹  James Schilling¹  Minnie M. Sarwal²  Li Li³ 

[1] Divisions of Immunology, Department of Pediatrics, Stanford University Medical School, Stanford University, Stanford, USADivisions of Immunology, Department of Pediatrics, Stanford University Medical School, Stanford University, Stanford, USADivisions of Immunology, Department of Pediatrics, Stanford University Medical School, Stanford University, Stanford, USA;Division of Nephrology, Department of Pediatrics, Stanford University Medical School, Stanford University, Stanford, USAStanford University Medical School, Stanford, USADivision of Nephrology, Department of Pediatrics, Stanford University Medical School, Stanford University, Stanford, USADivision of Nephrology, Department of Pediatrics, Stanford University Medical School, Stanford University, Stanford, USAStanford University Medical School, Stanford, USAStanford University Medical School, Stanford, USADivision of Nephrology, Department of Pediatrics, Stanford University Medical School, Stanford University, Stanford, USAStanford University Medical School, Stanford, USA;Division of Nephrology, Department of Pediatrics, Stanford University Medical School, Stanford University, Stanford, USADivision of Nephrology, Department of Pediatrics, Stanford University Medical School, Stanford University, Stanford, USADivision of Nephrology, Department of Pediatrics, Stanford University Medical School, Stanford University, Stanford, USA

关键词: Urine;    Peptidomics;    Biomarker;    Renal transplantation;    Proteomics;    LC MALDI;    Urinary Peptidomics;    Acute rejection;    Non-invasive biomarkers;    Biomarker discovery;   

DOI  :  10.1007/s12014-009-9029-0

来源: Humana Press Inc

【 摘 要 】

Abstract

Introduction

Human urine is a complex matrix of proteins, endogenous peptides, lipids, and metabolites. The level of any or all of these components can reflect the pathophysiological status of an individual especially of the kidney at the time of urine collection. The naturally occurring endogenous urinary peptides which are thought to be the product of several proteolytic and degradation processes may provide clinically useful biomarkers for different renal and systemic diseases.

Materials and Methods

To examine if specific differences in the urinary peptidome (<10 kDa) occur at the time of acute renal transplant rejection (AR), we undertook a study of urine samples collected from biopsy-proven AR (n = 10), stable graft function (n = 10), and healthy normal control (n = 10). The peptides (<10 kDa) were extracted and fractionated with high-performance liquid chromatography followed by matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometric (MS) analysis.

Results

We identified 54 endogenous peptides, including multiple peptides for Tamm–Horsfall protein (UMOD). A panel of peptides are identified which discriminate renal transplant patients with AR from stable graft. We have shown that liquid chromatography followed by MALDI is a useful tool to identify potential biomarkers, which after verification with larger patient cohort can be used as a non-invasive monitoring tool for renal transplant rejection.

【 授权许可】

Unknown