Tumor size, stage and grade alterations of urinary peptidome in RCC

Journal of Translational Medicine

Research

Marta Cazzaniga¹  Gabriele De Sio¹  Andrew James Smith¹  Fulvio Magni¹  Clizia Chinello¹  Giancarlo Mauri²  Italo Zoppis²  Stefano Signorini³  Silvano Bosari⁴  Marco Grasso⁵  Angelica Grasso⁶  Bernardo Rocco⁶ 

[1] Department of Health Science, School of Medicine, University of Milano-Bicocca (UNIMIB), Via Cadore, 48, 20900, Monza, Italy;Department of Informatics, Systems and Communication, University of Milano-Bicocca, Milan, Italy;Department of Laboratory Medicine, Hospital of Desio, Desio, Italy;Department of Medicine, Surgery and Dental Sciences, Pathology Unit, IRCCS-Policlinico Foundation, Mangiagalli and Regina Elena, University of Milan, Milan, Italy;Department of Surgical Pathology, Cytology, Medical Genetics and Nephropathology, Azienda Ospedaliera San Gerardo, Monza, Italy;Urology Unit, Department of Specialistic Surgical Sciences, Ospedale Maggiore Policlinico Foundation, Milan, Italy;

关键词: Mass spectrometry;    Proteomics;    Urine;    Renal cell carcinoma;    Tumour size;    Tumour progression;    Stage;    Grade;    Cancer;    Peptidomics;   

DOI  :  10.1186/s12967-015-0693-8

 received in 2015-07-17, accepted in 2015-10-10,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundSeveral promising biomarkers have been found for RCC, but none of them has been used in clinical practice for predicting tumour progression. The most widely used features for predicting tumour aggressiveness still remain the cancer stage, size and grade. Therefore, the aim of our study is to investigate the urinary peptidome to search and identify peptides whose concentrations in urine are linked to tumour growth measure and clinical data.MethodsA proteomic approach applied to ccRCC urinary peptidome (n = 117) based on prefractionation with activated magnetic beads followed by MALDI-TOF profiling was used. A systematic correlation study was performed on urinary peptide profiles obtained from MS analysis. Peptide identity was obtained by LC–ESI–MS/MS.ResultsFifteen, twenty-six and five peptides showed a statistically significant alteration of their urinary concentration according to tumour size, pT and grade, respectively. Furthermore, 15 and 9 signals were observed to have urinary levels statistically modified in patients at different pT or grade values, even at very early stages. Among them, C1RL, A1AGx, ZAG2G, PGBM, MMP23, GP162, ADA19, G3P, RSPH3, DREB, NOTC2 SAFB2 and CC168 were identified.ConclusionsWe identified several peptides whose urinary abundance varied according to tumour size, stage and grade. Among them, several play a possible role in tumorigenesis, progression and aggressiveness. These results could be a useful starting point for future studies aimed at verifying their possible use in the managements of RCC patients.

【 授权许可】

CC BY   
© Chinello et al. 2015