Proteomic Analysis of Two Non-Bronchoscopic Methods of Sampling the Lungs of Patients with the Acute Respiratory Distress Syndrome (ARDS)

Clinical Proteomics

Guido M. Domenighetti⁵  Jay Heinecke⁴  Shinichi Hayashi³  Gustavo Matute-Bello³  Trevor King¹  Thomas R. Martin²  Dong W. Chang³  Tomas Vaisar⁴  Roger Bumgarner¹  Giuseppe Colucci⁵ 

[1] Department of Medicine, University of Washington, Seattle, USADepartment of Microbiology, University of Washington, Seattle, USADepartment of Medicine, University of Washington, Seattle, USADepartment of Medicine, University of Washington, Seattle, USADepartment of Microbiology, University of Washington, Seattle, USADepartment of Microbiology, University of Washington, Seattle, USADepartment of Medicine, University of Washington, Seattle, USADepartment of Microbiology, University of Washington, Seattle, USA;Medical Research Service of the VA Puget Sound Healthcare System, University of Washington, Seattle, USADivisions of Pulmonary and Critical Care Medicine, University of Washington, Seattle, USASeattle VAMC Pulmonary Research Laboratories, VA Puget Sound Health Care System, Seattle, USAMedical Research Service of the VA Puget Sound Healthcare System, University of Washington, Seattle, USAMedical Research Service of the VA Puget Sound Healthcare System, University of Washington, Seattle, USADivisions of Pulmonary and Critical Care Medicine, University of Washington, Seattle, USASeattle VAMC Pulmonary Research Laboratories, VA Puget Sound Health Care System, Seattle, USADivisions of Pulmonary and Critical Care Medicine, University of Washington, Seattle, USAMedical Research Service of the VA Puget Sound Healthcare System, University of Washington, Seattle, USADivisions of Pulmonary and Critical Care Medicine, University of Washington, Seattle, USASeattle VAMC Pulmonary Research Laboratories, VA Puget Sound Health Care System, Seattle, USASeattle VAMC Pulmonary Research Laboratories, VA Puget Sound Health Care System, Seattle, USAMedical Research Service of the VA Puget Sound Healthcare System, University of Washington, Seattle, USADivisions of Pulmonary and Critical Care Medicine, University of Washington, Seattle, USASeattle VAMC Pulmonary Research Laboratories, VA Puget Sound Health Care System, Seattle, USA;Medical Research Service of the VA Puget Sound Healthcare System, University of Washington, Seattle, USADivisions of Pulmonary and Critical Care Medicine, University of Washington, Seattle, USAMedical Research Service of the VA Puget Sound Healthcare System, University of Washington, Seattle, USAMedical Research Service of the VA Puget Sound Healthcare System, University of Washington, Seattle, USADivisions of Pulmonary and Critical Care Medicine, University of Washington, Seattle, USADivisions of Pulmonary and Critical Care Medicine, University of Washington, Seattle, USAMedical Research Service of the VA Puget Sound Healthcare System, University of Washington, Seattle, USADivisions of Pulmonary and Critical Care Medicine, University of Washington, Seattle, USA;Division and Endocrinology and Metabolism, University of Washington, Seattle, USADivision and Endocrinology and Metabolism, University of Washington, Seattle, USADivision and Endocrinology and Metabolism, University of Washington, Seattle, USA;Multidisciplinary Intensive Care Unit, Regional Hospital, Locarno, SwitzerlandMultidisciplinary Intensive Care Unit, Regional Hospital, Locarno, SwitzerlandMultidisciplinary Intensive Care Unit, Regional Hospital, Locarno, Switzerland

关键词: Acute respiratory distress syndrome;    Bronchoalveolar lavage;    Bronchoscopy;    Lung injury;    Proteomics;   

DOI  :  10.1007/s12014-007-9002-8

来源: Humana Press Inc

【 摘 要 】

Abstract

Objective

The collection of lung fluid using a suction catheter (s-Cath) and non-bronchoscopic bronchoalveolar lavage (mini-BAL) are two minimally invasive methods of sampling the distal airspaces in patients with the acute respiratory distress syndrome (ARDS). The objective of this study was to determine the similarity of the lung fluid samples recovered by these methods using proteomic analysis.

Methods

Distal lung fluid samples were collected from seven mechanically ventilated patients with ARDS using both s-Cath and mini-BAL in each patient and compared using two-dimensional difference gel electrophoresis. Protein spots of interest were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Main Results

An average of 2,164 spots was detected in the s-Cath and mini-BAL samples. Of these, 68.4% of the protein spots were similar between the s-Cath and mini-BAL samples, 13.2% were increased in s-Cath compared to mini-BAL, and 18.4% were decreased in s-Cath compared to mini-BAL. For each of the seven subjects, overabundance analysis showed that the actual number of differentially expressed spots in the mini-BAL and s-Cath sample was more than the expected number if the samples were identical. There were nine proteins that were consistently differentially expressed between the mini-BAL and s-Cath samples. Of these nine proteins, five are abundantly found in neutrophils or airway epithelial cells, suggesting that the s-Cath may sample the bronchial airways to a greater extent than mini-BAL.

Conclusion

Proteomic analysis of mini-BAL and s-Cath samples shows for the first time that, although these two methods for sampling the lungs of critically ill patients are generally similar, the s-Cath method oversamples the distal airways compared to the mini-BAL method.

【 授权许可】

Unknown