The Inhibitory Effect of Nicotine on TNF- Expression in Human Fetal Astrocytes

Journal of the Korean Chemical Society

Hyun Duk Yang¹  Seung Han Suk¹  Il Hong Son¹  Jai Kyoo Lee¹  Sun Jung Han¹  Sung Ik Lee¹  Joo Young Park¹  Hyung-In Moon¹  Sung Soo Lee¹  Jae Hyun Kim¹ 

关键词: TNF-¥á;    Human fetal astrocytes;    nicotine;    NF-¥êB;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

The Tumor necrosis factor-¥á, (TNF-¥á), is involved in the pathogenesis of multiple sclerosis and contributes to the degeneration of oligodendrocytes as well as neurons. Nicotine has been found to have immunosuppressive and inflammation-suppressing effects. Astrocytes, the major glial cells in the CNS, are capable of producing TNF-¥á at both the mRNA and protein levels in response to interleukin-1 (IL-1) or TNF-¥á. Nicotine has been shown to influence glial cell functions. To order to explore the role of astrocytes in the production of TNF-¥á, astrocytes were pretreated with nicotine and are stimulated with IL-1¥â to determine their effects on TNF-¥á production. The results are as follows. Cytotoxic effects of nicotine on human fetal astrocytes were noted above 10 ¥ìg/ml of nicotine. The effect of IL-1¥â on TNF-¥á mRNA expression in primary cultured human fetal astrocytes was maximal at 2 h after IL- 1¥â(100 pg/ml) treatment. Human fetal astrocytes were pretreated with 0.1, 1, and 10 ¥ìg/ml of nicotine and then stimulated with IL-1¥â (100 pg/ml) for 2 h. The inhibitory effect of nicotine on expressions of TNF-¥á mRNA in human fetal astrocytes with pretreated 0.1 ¥ìg/ml of nicotine is first noted at 8 hr, and the inhibitory effect is maximal at 12 h. The inhibitory effect at 1 ¥ìg/ml of nicotine is inhibited maximal at 24 h. Nicotine at 0.1, 1 and 10 ¥ìg/ml concentrations significantly inhibits IL-1¥â-induced NF-¥êB activation. Collectively, this study indicates that nicotine might inhibit the expression of TNF-¥á in activated human fetal astrocytes.

【 授权许可】

Unknown