Analysis of Androgen Receptor Gene by Capillary Gel Eelectrophoresis

Journal of the Korean Chemical Society

Suman Lee¹  Chungmu Kang¹  Kyuseong Choi¹  Kiwha Chung¹  Yongseong Kim¹  Seungkwon Back¹  Daechun Kang¹  Byungok Choi¹ 

关键词: Capillary;    Electrophoresis;    CAG Repeat;    Infertile;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Capillary gel electrophoresis (CGE) with poly(ethylene oxide) has been applied to the measurement of CAG repeat number in Androgen receptor (AR) gene related to male infertility. Non-linear regression analysis was performed using the standard ?X174 RF/Hae III, 100bp step ladder DNA in order to investigate the exact number of CAG repeat. For 79 Korean infertile males and 89 controls, CAG repeats at exon 1 in AR gene was compared and analyzed by CGE. It turned out that CAG repeat numbers were 24.97¡¾2.6 range, 17-29) for the infertile male, and 23.99¡¾2.4 range, 18-29) for the control, respectively. P value (0.018) was less then 0.05, meaning that the result was statistically meaningful.

【 授权许可】

Unknown